@article{fdi:010061361,
  title = {{P}hylogenetic character mapping of {RADES} {P}robing, a new marker for exploring the clonal evolution of expressed coding sequences in {T}rypanosoma cruzi, the agent of {C}hagas disease},
  author = {{T}elleria, {J}enny and {B}arnab{\'e}, {C}hristian and {A}yala, {F}. {J}. and {T}ibayrenc, {M}ichel},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e have tested a new genetic marker, {RADES} {P}robing ({RADES}-{P}), on a standard sample of 19 laboratory-cloned stocks of {T}rypanosoma cruzi, the agent of {C}hagas disease. {T}his set of stocks, fully characterized using multilocus enzyme electrophoresis ({MLEE}) and random amplified polymorphic {DNA} ({RAPD}), is representative of this parasite's main genetic subdivisions. {RADES}-{P} consists in hybridizing {RAPD} profiles with probes composed of the products of random amplified differentially expressed sequences ({RADES}). {T}he profiles thus obtained uncover only expressed coding sequences that are as well present on {RAPD} gels. {D}irect visual examination and the banding record show that these {RADES}-{P} profiles are different of, and not redundant with, both {RAPD} and {RADES} patterns obtained on the same set of stocks with the same primers. {P}hylogenetic character mapping ({PCM}) of the {RADES}-{P} polymorphism fairly confirms the known population structure and phylogenetic diversity of {T}. cruzi. {T}his suggests that the impact of clonal evolution on {T}. cruzi has been predominant enough over the long term to carve the polymorphism of all types of {DNA} sequences, including polymorphisms of expressed coding sequences, although these sequences are subject to natural selection.},
  keywords = {{G}enetic marker ; {P}arasite ; {I}nfectious disease ; {P}hylogeny ; {H}ybridization ; {DNA} probe ; {AMERIQUE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {19},
  numero = {},
  pages = {287--291},
  ISSN = {1567-1348},
  year = {2013},
  DOI = {10.1016/j.meegid.2013.03.027},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061361},
}