@article{fdi:010061336,
  title = {{D}ifferential protein expression profiles between plasmodium falciparum parasites isolated from subjects presenting with pregnancy-associated malaria and uncomplicated malaria in {B}enin},
  author = {{B}ertin, {G}. {I}. and {S}abbagh, {A}. and {G}uillonneau, {F}. and {G}uemouri, {S}ayeh and {E}zinmegnon, {S}. and {F}ederici, {C}. and {H}ounkpatin, {B}. and {F}ievet, {N}adine and {D}eloron, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {P}lasmodium falciparum is responsible for severe malaria, including pregnancy-associated malaria ({PAM}). {D}uring intra-erythrocytic maturation, the infected erythrocyte (i{E}) membrane is modified by insertion of parasite-derived proteins, primarily consisting of variant surface antigens such as {P}. falciparum erythrocyte membrane protein-1. {M}ethods. {T}o identify new {PAM}-specific parasite membrane proteins, we conducted a mass spectrometry-based proteomic study and compared the protein expression profiles of 10 {PAM} and 10 uncomplicated malaria ({UM}) samples. {R}esults. {W}e focused on the 454/1139 membrane-associated and hypothetical proteins for comparative analysis. {U}sing filter-based feature-selection methods combined with supervised data analysis, we identified a subset of 53 proteins that distinguished {PAM} and {UM} samples. {U}p to 19/20 samples were correctly assigned to their respective clinical group. {A} hierarchical clustering analysis of these 53 proteins based on the similarity of their expression profiles revealed 2 main clusters of 40 and 13 proteins that were under-or over-expressed, respectively, in {PAM}. {C}onclusions. {VAR}2{CSA} is identified and associated with {PAM}, validating our experimental approach. {O}ther {PAM}-predictive proteins included {PFI}1785w, {PF}14_0018, {PFB}0115w, {PFF}0325c, and {PFA}_0410w. {T}hese proteomics data demonstrate the involvement of selected proteins in the pathophysiology of {PAM}, providing new insights for the definition of potential new targets for a vaccine against {PAM}.},
  keywords = {mass spectrometry ; {P}lasmodium falciparum ; pregnancy-associated malaria ; field isolate ; protein identification ; protein abundance ; {BENIN}},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases},
  volume = {208},
  numero = {12},
  pages = {1987--1997},
  ISSN = {0022-1899},
  year = {2013},
  DOI = {10.1093/infdis/jit377},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061336},
}