Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

 

Publications des scientifiques de l'IRD

Bertin G. I., Sabbagh A., Guillonneau F., Guemouri Sayeh, Ezinmegnon S., Federici C., Hounkpatin B., Fievet Nadine, Deloron Philippe. (2013). Differential protein expression profiles between plasmodium falciparum parasites isolated from subjects presenting with pregnancy-associated malaria and uncomplicated malaria in Benin. Journal of Infectious Diseases, 208 (12), 1987-1997. ISSN 0022-1899

Accès réservé (Intranet IRD) Demander le PDF

Lien direct chez l'éditeur doi:10.1093/infdis/jit377

Titre
Differential protein expression profiles between plasmodium falciparum parasites isolated from subjects presenting with pregnancy-associated malaria and uncomplicated malaria in Benin
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000327544600009
AuteursBertin G. I., Sabbagh A., Guillonneau F., Guemouri Sayeh, Ezinmegnon S., Federici C., Hounkpatin B., Fievet Nadine, Deloron Philippe.
SourceJournal of Infectious Diseases, 2013, 208 (12), p. 1987-1997. ISSN 0022-1899
RésuméBackground. Plasmodium falciparum is responsible for severe malaria, including pregnancy-associated malaria (PAM). During intra-erythrocytic maturation, the infected erythrocyte (iE) membrane is modified by insertion of parasite-derived proteins, primarily consisting of variant surface antigens such as P. falciparum erythrocyte membrane protein-1. Methods. To identify new PAM-specific parasite membrane proteins, we conducted a mass spectrometry-based proteomic study and compared the protein expression profiles of 10 PAM and 10 uncomplicated malaria (UM) samples. Results. We focused on the 454/1139 membrane-associated and hypothetical proteins for comparative analysis. Using filter-based feature-selection methods combined with supervised data analysis, we identified a subset of 53 proteins that distinguished PAM and UM samples. Up to 19/20 samples were correctly assigned to their respective clinical group. A hierarchical clustering analysis of these 53 proteins based on the similarity of their expression profiles revealed 2 main clusters of 40 and 13 proteins that were under-or over-expressed, respectively, in PAM. Conclusions. VAR2CSA is identified and associated with PAM, validating our experimental approach. Other PAM-predictive proteins included PFI1785w, PF14_0018, PFB0115w, PFF0325c, and PFA_0410w. These proteomics data demonstrate the involvement of selected proteins in the pathophysiology of PAM, providing new insights for the definition of potential new targets for a vaccine against PAM.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Santé : généralités [050]
Descr. géo.BENIN
LocalisationFonds IRD [F B010061336] ; Montpellier (Centre IRD)
Identifiant IRDfdi:010061336
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010061336

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Cayenne

Nouméa

Papeete

Abidjan

Dakar

Niamey

Ouagadougou

Tunis

La Paz

Quito