@article{fdi:010061277,
  title = {{I}n vitro lipid peroxidation of intestinal bile salt-based nanoemulsions : potential role of antioxidants},
  author = {{C}ourraud, {J}ulie and {C}harnay, {C}. and {C}ristol, {J}. {P}. and {B}erger, {J}acques and {A}vallone, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ver the last decades, oxidative stress has been described as a deleterious phenomenon contributing to numerous noncommunicable diseases such as cardiovascular disease, diabetes, and cancers. {A}s many authors ascribed the healthy effect of fruit and vegetable consumption mainly to their antioxidant contents, it has been hypothesized that their protection could occur from the gut. {T}herefore, the aim of this study was to develop an original and physiological model of nanoemulsions to study lipid peroxidation within the intestine and to assess the properties of potential antioxidants in this setting. {S}everal nanoemulsions were compared in terms of physical characteristics and reactivity to 2,2'-azobis-(2-amidinopropane) hydrochloride ({AAPH})-induced oxidation. {F}ormulations included different types of lipids, a detergent (a conjugated bile salt or sodium dodecyl sulfate) and, finally, lipophilic antioxidants. {H}emin and myoglobin were also tested as relevant potential oxidants. {F}atty acid ({FA}) peroxidation was monitored by gas chromatography while malondialdehyde and antioxidant contents were measured by {HPLC}. {I}nvestigated nanoemulsions were composed of spherical or cylindrical mixed micelles, the latter being the least resistant to oxidation. {I}n the experimental conditions, {AAPH} was the only efficient oxidant. {A}lpha-tocopherol and lutein significantly slowed {FA} degradation from 4 to 1 mu {M}, respectively. {O}n the contrary, beta-carotene did not show any protective capacity at 4 mu {M}. {I}n conclusion, the tested nanoemulsions were appropriate to assess antioxidant capacity during the intestinal phase of digestion.},
  keywords = {micelles ; postprandial oxidative stress ; detergent ; {AAPH} ; tocopherol ; carotenoids ; digestion},
  booktitle = {},
  journal = {{F}ree {R}adical {R}esearch},
  volume = {47},
  numero = {12},
  pages = {1076--1087},
  ISSN = {1071-5762},
  year = {2013},
  DOI = {10.3109/10715762.2013.853877},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061277},
}