@article{fdi:010061226, title = {{M}olecular diversity of the {P}lanctomycetes in the human gut microbiota in {F}rance and {S}enegal}, author = {{C}ayrou, {C}. and {S}ambe, {B}. and {A}rmougom, {F}abrice and {R}aoult, {D}idier and {D}rancourt, {M}.}, editor = {}, language = {{ENG}}, abstract = {{U}ntil now, {P}lanctomycetes bacteria were considered as environmental organisms. {N}evertheless, some studies detected {P}lanctomycetes {DNA} from human gut. {W}e therefore explored the human gut {P}lanctomycetes content. {P}lanctomycetes-specific {PCR} primers were designed to amplify a 240-bp 16{S} r{RNA} gene fragment in human stool specimens from individuals in {F}rance and in {S}enegal and from endocarditis patients receiving antibiotics in {F}rance. {PCR} products were then cloned and sequenced. {PCR} detection revealed a significantly higher prevalence (1.8% vs 0.4%, p=0.05) and higher diversity (62 vs 6 phylotypes, p=0.02) of {P}lanctomycetes 16{S} r{RNA} gene in stool specimens collected in {S}enegal than in {F}rance. {A}lso, stool specimens from endocarditis patients exhibited non-significantly higher prevalence (0.6% vs 0.4%) and the ratio of phylotypes by positive patient (3 vs 1.5) than those collected from untreated {F}rench individuals. {G}emmata sp. related sequences were found in 6/12 individuals. {P}lanctomycetes organisms are a part of the human digestive tract microbiota. {T}heir diversity varied by environment including the geographical origin of the individual and antibiotics treatment.}, keywords = {{D}etection ; diversity ; human gut ; {P}lanctomycetes ; {FRANCE} ; {SENEGAL}}, booktitle = {}, journal = {{A}pmis}, volume = {121}, numero = {11}, pages = {1082--1090}, ISSN = {0903-4641}, year = {2013}, DOI = {10.1111/apm.12087}, URL = {https://www.documentation.ird.fr/hor/fdi:010061226}, }