@article{fdi:010061224,
  title = {{D}ifferential adhesion-inhibitory patterns of antibodies raised against two major variants of the {NTS}-{DBL}2{X} region of {VAR}2{CSA}},
  author = {{D}oritchamou, {J}. and {B}igey, {P}. and {N}ielsen, {M}. {A}. and {G}nidehou, {S}. and {E}zinmegnon, {S}. and {B}urgain, {A}. and {M}assougbodji, {A}. and {D}eloron, {P}hilippe and {S}alanti, {A}. and {T}uikue {N}dam, {N}icaise},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {VAR}2{CSA} is a large polymorphic {P}lasmodium falciparum protein expressed on infected erythrocytes ({IE}) that allows their binding in the placenta, thus precipitating placental malaria ({PM}). {T}he {N}-terminal part of {VAR}2{CSA} that contains the binding site to placental chondroitin sulfate {A} ({CSA}) is currently recognized as the most attractive region for vaccine development. {A}n ultimate challenge is to define epitopes in this region that induce a broad cross-reactive adhesion inhibitory antibody response. {M}ethods: {B}ased on phylogenetic data that identified a dimorphic sequence motif in the {VAR}2{CSA} {DBL}2{X}, we raised antibodies against the {NTS}-{DBL}2{X} constructs containing one sequence or the other (3{D}7 and {FCR}3) and tested their functional properties on {P}. falciparum isolates from pregnant women and on laboratory-adapted strains. {R}esults: {T}he {CSA} binding inhibitory capacity of the antibodies induced varied from one parasite isolate to another (range, 10%-100%), but the combined analysis of individual activity highlighted a broader functionality that increased the total number of isolates inhibited. {I}nterestingly, the differential inhibitory effect of the antibodies observed on field isolates resulted in significant inhibition of all field isolates tested, suggesting that optimal inhibitory spectrum on field isolates from pregnant women might be achieved with antibodies targeting limited variants of the {N}-terminal {VAR}2{CSA}. {C}onclusions: {O}ur findings indicate that the {NTS}-{DBL}2{X} region of {VAR}2{CSA} can elicit strain-transcending anti-adhesion antibodies and suggest that the combination of the two major variants used here could represent the basis for an effective bivalent {VAR}2{CSA}-based vaccine.},
  keywords = {{P}lasmodium falciparum ; {P}regnancy ; {M}alaria ; {VAR}2{CSA} ; {V}accine},
  booktitle = {},
  journal = {{V}accine},
  volume = {31},
  numero = {41},
  pages = {4516--4522},
  ISSN = {0264-410{X}},
  year = {2013},
  DOI = {10.1016/j.vaccine.2013.07.072},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061224},
}