@article{fdi:010061221,
  title = {{C}ulturomics identified 11 new bacterial species from a single anorexia nervosa stool sample},
  author = {{P}fleiderer, {A}. and {L}agier, {J}. {C}. and {A}rmougom, {F}abrice and {R}obert, {C}. and {V}ialettes, {B}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he rebirth of bacterial culture has been highlighted successively by environmental microbiologists, the design of axenic culture for intracellular bacteria in clinical microbiology, and, more recently, by human gut microbiota studies. {I}ndeed, microbial culturomics (large scale of culture conditions with the identification of colonies by {MALDI}-{TOF} or 16{S} r{RNA}) allowed to culture 32 new bacterial species from only four stool samples studied. {W}e performed culturomics in comparison with pyrosequencing 16{S} r{RNA} targeting the {V}6 region on an anorexia nervosa stool sample because this clinical condition has never been explored before by culture, while its composition has been observed to be atypical by metagenomics. {W}e tested 88 culture conditions generating 12,700 different colonies identifying 133 bacterial species, with 19 bacterial species never isolated from the human gut before, including 11 new bacterial species for which the genome has been sequenced. {T}hese 11 new bacterial species isolated from a single stool sample allow to extend more significantly the repertoire in comparison to the bacterial species validated by the rest of the world during the last 2 years. {P}yrosequencing indicated a dramatic discrepancy with the culturomics results, with only 23 {OTU}s assigned to the species level overlapping (17 % of the culturomics results). {M}ost of the sequences assigned to bacteria detected only by pyrosequencing belonged to {R}uminococcaceae, {L}achnospiraceae, and {E}rysipelotrichaceae constituted by strictly anaerobic species, indicating the future route for culturomics. {T}his study revealed new bacterial species participating significantly to the extension of the gut microbiota repertoire, which is the first step before being able to connect the bacterial composition with the geographic or clinical status.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {32},
  numero = {11},
  pages = {1471--1481},
  ISSN = {0934-9723},
  year = {2013},
  DOI = {10.1007/s10096-013-1900-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061221},
}