@article{fdi:010061183,
  title = {{E}xpression of the domain cassette 8 plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in {B}enin},
  author = {{B}ertin, {G}. {I}. and {L}avstsen, {T}. and {G}uillonneau, {F}. and {D}oritchamou, {J}. and {W}ang, {C}. {W}. and {J}espersen, {J}. {S}. and {E}zimegnon, {S}. and {F}ievet, {N}adine and {A}lao, {M}. {J}. and {L}alya, {F}. and {M}assougbodji, {A}. and {T}uikue {N}dam, {N}icaise and {T}heander, {T}. {G}. and {D}eloron, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}lasmodium falciparum erythrocyte membrane protein-1 ({P}f{EMP}-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. {B}ased on sequence homology {P}f{EMP}-1 variants have been grouped into three major groups {A}-{C}, the highly conserved {VAR}2{CSA} variants, and semi-conserved types defined by tandem runs of specific domains ("domain cassettes' ({DC})). {T}he {P}f{EMP}-1 type expressed determines the adherence phenotype, and is associated with clinical outcome of infection. {M}ethods: {P}arasite isolates from {B}eninese children or women presenting with, respectively, {CM} or {PAM} were collected along with samples from patients with uncomplicated malaria ({UM}). {W}e assessed the transcript level of var genes by {RT}-q{PCR} and the expression of {P}f{EMP}-1 proteins by {LC}-{MS}/{MS}. {R}esults: {V}ar genes encoding {DC}8 and {G}roup {A} {P}f{EMP}-1 were transcribed more often and at higher levels in cerebral malaria vs. uncomplicated malaria patients. {LC}-{MS}/{MS} identified peptides from group {A}, {DC}8 {P}f{EMP}-1 more frequently in cerebral malaria than in uncomplicated malaria and pregnancy-associated malaria samples. {C}onclusion: {T}his is the first study to show association between {P}f{EMP}-1 subtype and disease outcome by direct analysis of parasites proteome. {T}he results corroborate that group {A} and specifically the {P}f{EMP}-1 types {DC}8 are universally associated with cerebral malaria. {T}his is a crucial observation for promoting studies on malaria pathogenesis.},
  keywords = {{BENIN}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {7},
  pages = {e68368},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0068368},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061183},
}