@article{fdi:010061176,
  title = {{I}nvestigating the genetics of {B}ti resistance using m{RNA} tag sequencing : application on laboratory strains and natural populations of the dengue vector {A}edes aegypti},
  author = {{P}aris, {M}. and {M}arcombe, {S}. and {C}oissac, {E}. and {C}orbel, {V}incent and {D}avid, {J}. {P}. and {D}espres, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}osquito control is often the main method used to reduce mosquito-transmitted diseases. {I}n order to investigate the genetic basis of resistance to the bio-insecticide {B}acillus thuringiensis subsp. israelensis ({B}ti), we used information on polymorphism obtained from c{DNA} tag sequences from pooled larvae of laboratory {B}ti-resistant and susceptible {A}edes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms ({SNP}s). {O}f the 372 {SNP}s tested, 99.2% were validated using {DNA} {I}llumina {G}olden{G}ate(({R})) array, with a strong correlation between the allelic frequencies inferred from the pooled and individual data (r=0.85). {A} total of 11 genomic regions and five candidate genes were detected using a genome scan approach. {O}ne of these candidate genes showed significant departures from neutrality in the resistant strain at sequence level. {S}ix natural populations from {M}artinique {I}sland were sequenced for the 372 tested {SNP}s with a high transferability (87%), and association mapping analyses detected 14 loci associated with {B}ti resistance, including one located in a putative receptor for {C}ry11 toxins. {T}hree of these loci were also significantly differentiated between the laboratory strains, suggesting that most of the genes associated with resistance might differ between the two environments. {I}t also suggests that common selected regions might harbour key genes for {B}ti resistance.},
  keywords = {{A}edes aegypti ; {B}acillus thurigiensis var israelensis ; genome scan ; insecticide resistance ; next-generation sequencing ; {RNA}seq ; {MARTINIQUE}},
  booktitle = {},
  journal = {{E}volutionary {A}pplications},
  volume = {6},
  numero = {7},
  pages = {1012--1027},
  ISSN = {1752-4571},
  year = {2013},
  DOI = {10.1111/eva.12082},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061176},
}