@article{fdi:010061167,
  title = {{N}onhuman primate retroviruses from {C}ambodia : high simian foamy virus prevalence, identification of divergent {STLV}-1 strains and no evidence of {SIV} infection},
  author = {{A}youba, {A}hidjo and {D}uval, {L}. and {L}i{\'e}geois, {F}lorian and {N}gin, {S}. and {A}huka-{M}undeke, {S}. and {S}witzer, {W}. {M}. and {D}elaporte, {E}ric and {A}riey, {F}. and {P}eeters, {M}artine and {N}errienet, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}onhuman primates ({NHP}s) carry retroviruses such as simian immunodeficiency viruses ({S}ly), simian {T}-cell lymphotropic viruses ({STLV}) and simian foamy viruses ({SFV}). {H}ere, we revisited {NHP}s from {C}ambodia to assess the prevalence and diversity of these retroviruses using updated viral detection tools. {W}e screened blood from 118 {NHP}s consisting of six species ({M}acaca fascicularis (n = 91), {M}acaca leonine (n = 8), {P}resbytis cristata (n = 3), {N}ycticebus coucang (n = 1), {H}ylobates pileatus (n = 14), and {P}ongo pygmaeus) (n = 1) by using a {L}uminex-based multiplex serology assay that allows the detection of all known {SIV}/{HIV} and {SFV} lineages. {W}e also used highly sensitive {PCR} assays to detect each simian retrovirus group. {P}ositive {PCR} products were sequenced and phylogenetically analyzed to infer evolutionary histories. {F}ifty-three of 118 (44.9%) {NHP}s tested positive for {SFV} by serology and 8/52 (15.4%), all from {M}. fascicularis, were {PCR}-confirmed. {T}he 8 novel {SFV} sequences formed a highly supported distinct lineage within a clade composed of other macaque {SFV}. {W}e observed no serological or molecular evidence of {S}ly infection among the 118 {NHP} samples tested. {F}our of 118 (3.3%) {NHP}s were {PCR}-positive for {STLV}, including one {M}. fascicularis, one {P}. cristata, and two {H}. pileatus. {P}hylogenetic analyses revealed that the four novel {STLV} belonged to the {PTLV}-1 lineage, outside the {A}frican radiation of {PTLV}-1, like all {A}sian {PTLV} identified so far. {S}equence analysis of the whole {STLV}-1 genome from a {H}. pileatus ({C}578_{H}p) revealed a genetic structure characteristic of {PTLV}. {S}imilarity analysis comparing the {STLV}-1 ({C}578_{H}p) sequence with prototype {PTLV}s showed that {C}578_{H}p is closer to {PTLV}-ls than to all other types across the entire genome. {I}n conclusion, we showed a high frequency of {SFV} infection but found no evidence of {SIV} infection in {NHP}s from {C}ambodia. {W}e identified for the first time {STLV}-1 in a {P}. cristata and in two {H}. pileatus.},
  keywords = {{N}onhuman primates ; {R}etroviruses ; {C}ambodia ; {SIV} ; {STLV} ; {SFV} ; {CAMBODGE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {18},
  numero = {},
  pages = {325--334},
  ISSN = {1567-1348},
  year = {2013},
  DOI = {10.1016/j.meegid.2013.04.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061167},
}