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Dama E., Cornélie Sylvie, Camara M., Somda M. B., Poinsignon Anne, Ilboudo H., Ndille E. E., Jamonneau Vincent, Solano Philippe, Remoué Franck, Bengaly Z., Belem A. M. G., Bucheton Bruno. (2013). In silico identification of a candidate synthetic peptide (Tsgf1(18-43)) to monitor human exposure to tsetse flies in West Africa. Plos Neglected Tropical Diseases, 7 (9), e2455. ISSN 1935-2735

Fichier PDF disponiblehttp://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers15-10/010061163.pdf[ PDF Link ]

Lien direct chez l'éditeur doi:10.1371/journal.pntd.0002455

Titre
In silico identification of a candidate synthetic peptide (Tsgf1(18-43)) to monitor human exposure to tsetse flies in West Africa
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000324920800054
AuteursDama E., Cornélie Sylvie, Camara M., Somda M. B., Poinsignon Anne, Ilboudo H., Ndille E. E., Jamonneau Vincent, Solano Philippe, Remoué Franck, Bengaly Z., Belem A. M. G., Bucheton Bruno.
SourcePlos Neglected Tropical Diseases, 2013, 7 (9), p. e2455. p. e2455 ISSN 1935-2735
RésuméBackground: The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals. Methodology/Principal Findings: Synthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf1(18-43) specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p < 0.0001) in the two populations exposed to tsetse flies (Guinean HAT foci, and South West Burkina Faso). A positive correlation was also found between Anti-Tsgf1(18-43) IgG levels and the risk of being infected by Trypanosoma brucei gambiense in the sleeping sickness foci of Guinea. Conclusion/Significance: The Tsgf1(18-43) peptide is a suitable and promising candidate to develop a standardize immunoassay allowing large scale monitoring of human exposure to tsetse flies in West Africa. This could provide a new surveillance indicator for tsetse control interventions by HAT control programs.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
Descr. géo.AFRIQUE DE L'OUEST
LocalisationFonds IRD [F B010061163]
Identifiant IRDfdi:010061163
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010061163

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