@article{fdi:010061155,
  title = {{I}dentification and genetic characterization of {T}rypanosoma congolense in domestic animals of {F}ontem in the {S}outh-{W}est region of {C}ameroon},
  author = {{S}imo, {G}. and {S}obgwi, {P}. {F}. and {N}jitchouang, {G}. {R}. and {N}jiokou, {F}. and {K}uiate, {J}. {R}. and {C}uny, {G}{\'e}rard and {A}songanyi, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o understand the circulation and the spread of {T}orpanosoma congolense genotypes in animals of {F}ontem in the southwest region of {C}ameroon, {T}. congolense forest and {T}. congolense savannah were investigated in 397 domestic animals in eight villages. {O}ut of the 397 domestic animals, 86 (21.7%) were found infected by trypanosomes, using the capillary tube centrifugation test. {T}he {PCR} with specific primers identified 163 (41.1%) and 81(20.4%) animals infected by {T}. congolense forest and {T}. congolense savannah, respectively; showing for the first time the circulation of {T}. congolense savannah in the {F}ontem region. {N}o infection with {T}. congolense savannah was found in pigs whereas goats and sheep were infected by {T}. congolense forest and/or {T}. congolense savannah. {T}he prevalence of trypanosomes varied significantly amongst villages and animal species. {T}he genotyping of {T}. congolense forest positive samples using microsatellites markers showed that multiple genotypes occurred in 27.2% (44/163) of animals sampled, whereas single genotypes were found in 73.8% (119/163) of samples. {S}ome alleles were found in all animal species as well as in all villages and were responsible for major genotypes, whereas others (rare alleles) were identified only in some animals of few villages. {T}hese rare alleles were characteristic of specific genotypes, assimilated to minor genotypes which can be spread in the region through tsetse flies. {T}he microsatellite markers show a low genetic variability and an absence of sub-structuration within {T}. congolense forest. {T}he analysis of the microsatellite data revealed a predominant clonal reproduction within {T}. congolense forest. {P}igs were the animal species with the highest number of different genotypes of {T}. congolense forest. {T}hey seem to play an important epidemiological role in the propagation and spread of different genotypes of {T}. congolense.},
  keywords = {{T}rypanosoma congolense ; {M}icrosatellite ; {G}enotypes ; {A}nimal {A}frican ; trypanosomiasis ; {CAMEROUN}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {18},
  numero = {},
  pages = {66--73},
  ISSN = {1567-1348},
  year = {2013},
  DOI = {10.1016/j.meegid.2013.04.019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061155},
}