@article{fdi:010061133,
  title = {{A} {L}i{T}at 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for {T}rypanosoma brucei gambiense},
  author = {{V}an {B}ieuwenhove, {L}. and {B}uscher, {P}. and {B}alharbi, {F}. and {H}umbert, {M}. and {G}uizez, {Y}. and {L}ejon, {V}eerle},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {T}o evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein ({VSG}) {L}i{T}at 1.5 amino acid ({AA}) sequence 268–281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of {T}rypanosoma brucei gambiense sleeping sickness. {M}ethods: {A} synthetic biotinylated peptide (peptide 1.5/268–281), native {VSG} {L}i{T}at 1.3 and {VSG} {L}i{T}at 1.5 were tested in an indirect {ELISA} with 102 sera from patients with {HAT} and 102 endemic {HAT}-negative controls. {R}esults: {T}he area under the curve ({AUC}) of peptide 1.5/268–281 was 0.954 (95% confidence interval 0.918–0.980), indicating diagnostic potential. {T}he areas under the curve of {VSG} {L}i{T}at 1.3 and {L}i{T}at 1.5 were 1.000 (0.982–1.000) and 0.997 (0.973–1.000), respectively, and significantly higher than the {AUC} of peptide 1.5/268–281. {O}n a model of {VSG} {L}i{T}at 1.5, peptide 1.5/268–281 was mapped near the top of the {VSG}. {C}onclusions: {A} biotinylated peptide corresponding to {AA} 268–281 of {VSG} {L}i{T}at 1.5 may replace the native {VSG} in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native {VSG} {L}i{T}at 1.3 and {VSG} {L}i{T}at 1.5.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {18},
  numero = {4},
  pages = {461--465},
  ISSN = {0002-9637},
  year = {2013},
  DOI = {10.1111/tmi.12058},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061133},
}