@article{fdi:010061130,
  title = {{D}iagnostic accuracy of {L}oopamp {T}rypanosoma brucei detection kit for diagnosis of human {A}frican trypanosomiasis in clinical samples},
  author = {{M}itashi, {P}. and {H}asker, {E}. and {M}oumba {N}goyi, {D}. and {P}atient {P}yana, {P}. and {L}ejon, {V}eerle and {V}an der {V}eken, {W}. and {L}utumba, {P}. and {B}oelaert, {M}. and {D}eborggraeve, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}olecular methods have great potential for sensitive parasite detection in the diagnosis of human {A}frican trypanosomiasis ({HAT}), but the requirements in terms of laboratory infrastructure limit their use to reference centres. {A} recently developed assay detects the {T}rypanozoon repetitive insertion mobile element ({RIME}) {DNA} under isothermal amplification conditions and has been transformed into a ready-to-use kit format, the {L}oopamp {T}rypanosoma brucei. {I}n this study, we have evaluated the diagnostic performance of the {L}oopamp {T}rypanosoma brucei assay (hereafter called {LAMP}) in confirmed {T}.b. gambiense {HAT} patients, {HAT} suspects and healthy endemic controls from the {D}emocratic {R}epublic of the {C}ongo ({DRC}). {M}ethodology/{P}rincipal findings: 142 {T}.b. gambiense {HAT} patients, 111 healthy endemic controls and 97 {HAT} suspects with unconfirmed status were included in this retrospective evaluation. {R}eference standard tests were parasite detection in blood, lymph or cerebrospinal fluid. {A}rchived {DNA} from blood of all study participants was analysed in duplicate with {LAMP}. {S}ensitivity of {LAMP} in parasitologically confirmed cases was 87.3% (95% {CI} 80.9–91.8%) in the first run and 93.0% (95% {CI} 87.5–96.1%) in the second run. {S}pecificity in healthy controls was 92.8% (95% {CI} 86.4–96.3%) in the first run and 96.4% (95% {CI} 91.1–98.6%) in the second run. {R}eproducibility was excellent with a kappa value of 0.81. {C}onclusions/{S}ignificance: {I}n this laboratory-based study, the {L}oopamp {T}rypanosoma brucei {D}etection {K}it showed good diagnostic accuracy and excellent reproducibility. {F}urther studies are needed to assess the feasibility of its routine use for diagnosis of {HAT} under field conditions.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {7},
  numero = {10},
  pages = {e2504 [5  en ligne]},
  ISSN = {1935-2727},
  year = {2013},
  DOI = {10.1371/journal.pntd.0002504},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061130},
}