@article{fdi:010060893,
  title = {{I}dentification of the {H}evea brasiliensis {AP}2/{ERF} superfamily by {RNA} sequencing},
  author = {{D}uan, {C}. {F}. and {A}rgout, {X}. and {G}ebelin, {V}. and {S}ummo, {M}. and {D}ufayard, {J}. {F}. and {L}eclercq, {J}. and {K}uswanhadi, and {P}iyatrakul, {P}. and {P}irrello, {J}. and {R}io, {M}. and {C}hampion, {A}ntony and {M}ontoro, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {R}ubber tree ({H}evea brasiliensis) laticifers are the source of natural rubber. {R}ubber production depends on endogenous and exogenous ethylene (ethephon). {AP}2/{ERF} transcription factors, and especially {E}thylene-{R}esponse {F}actors, play a crucial role in plant development and response to biotic and abiotic stresses. {T}his study set out to sequence transcript expressed in various tissues using next-generation sequencing and to identify {AP}2/{ERF} superfamily in the rubber tree. {R}esults: {T}he 454 sequencing technique was used to produce five tissue-type transcript libraries (leaf, bark, latex, embryogenic tissues and root). {R}eads from all libraries were pooled and reassembled to improve m{RNA} lengths and produce a global library. {O}ne hundred and seventy-three {AP}2/{ERF} contigs were identified by in silico analysis based on the amino acid sequence of the conserved {AP}2 domain from the global library. {T}he 142 contigs with the full {AP}2 domain were classified into three main families (20 {AP}2 members, 115 {ERF} members divided into 11 groups, and 4 {RAV} members) and 3 soloist members. {F}ifty-nine {AP}2/{ERF} transcripts were found in latex. {A}longside the micro{RNA}172 already described in plants, eleven additional micro{RNA}s were predicted to inhibit {H}evea {AP}2/{ERF} transcripts. {C}onclusions: {H}evea has a similar number of {AP}2/{ERF} genes to that of other dicot species. {W}e adapted the alignment and classification methods to data from next-generation sequencing techniques to provide reliable information. {W}e observed several specific features for the {ERF} family. {T}hree {H}b{S}oloist members form a group in {H}evea. {S}everal {AP}2/{ERF} genes highly expressed in latex suggest they have a specific function in {H}evea. {T}he analysis of {AP}2/{ERF} transcripts in {H}evea presented here provides the basis for studying the molecular regulation of latex production in response to abiotic stresses and latex cell differentiation.},
  keywords = {{E}thylene ; {R}ubber ; {T}ranscription factor ; {T}ranscriptome},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {14},
  numero = {},
  pages = {30},
  ISSN = {1471-2164},
  year = {2013},
  DOI = {10.1186/1471-2164-14-30},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060893},
}