Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

 

Publications des scientifiques de l'IRD

Dubourg G., Lagier J. C., Armougom Fabrice, Robert C., Hamad I., Brouqui P., Raoult Didier. (2013). The gut microbiota of a patient with resistant tuberculosis is more comprehensively studied by culturomics than by metagenomics. European Journal of Clinical Microbiology and Infectious Diseases, 32 (5), 637-645. ISSN 0934-9723

Accès réservé (Intranet IRD) Demander le PDF

Lien direct chez l'éditeur doi:10.1007/s10096-012-1787-3

Titre
The gut microbiota of a patient with resistant tuberculosis is more comprehensively studied by culturomics than by metagenomics
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000317427100007
AuteursDubourg G., Lagier J. C., Armougom Fabrice, Robert C., Hamad I., Brouqui P., Raoult Didier.
SourceEuropean Journal of Clinical Microbiology and Infectious Diseases, 2013, 32 (5), p. 637-645. ISSN 0934-9723
RésuméGut microbiota consists of 10(10) bacteria per gram of stool. Many antibiotic regimens induce a reduction in both the diversity and the abundance of the gut flora. We analyzed one stool sample collected from a patient treated for drug-resistant Mycobacterium tuberculosis and who ultimately died from pneumonia due to a Streptococcus pneumoniae 10 months later. We performed microscopic observation, used 70 culture conditions (microbial culturomics) with identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and 16S rRNA amplification and sequencing, pyrosequencing, and 18S rRNA amplification and clone sequencing. Electron and optical microscopic observations revealed the presence of yeast, but no bacterial species were observed. By culture, only 39 bacterial species were identified, including one new species, as well as three species that have not been previously observed in the human gut. The pyrosequencing showed only 18 phylotypes, detecting a lower number of bacterial species than the culture techniques. Only two phylotypes overlapped with culturomics. In contrast, an amount of chloroplasts was found. Additionally, specific molecular eukaryote detection found three fungal species. We recovered, for the first time, more cultivable than non-cultivable bacterial species in a patient with a low bacterial load in the gut, demonstrating the depth bias of pyrosequencing. We propose that the desertification of gut microbiota in this patient is a reflection of the total body microbiota and may have contributed to the invasive infection of S. pneumoniae. This finding suggests that caution should be applied when treating patients with broad-spectrum antibiotics, and preventive measures should be taken in order to avoid invasive infection.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Biotechnologies [084]
LocalisationFonds IRD [F B010060809]
Identifiant IRDfdi:010060809
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010060809

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Cayenne

Nouméa

Papeete

Abidjan

Dakar

Niamey

Ouagadougou

Tunis

La Paz

Quito