%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Amziane, M.
%A Metiaz, F.
%A Darenfed-Bouanane, A.
%A Djenane, Z.
%A Selama, O.
%A Abderrahmani, A.
%A Cayol, J. L.
%A Fardeau, Marie-Laure
%T Virgibacillus natechei sp nov., a moderately halophilic bacterium isolated from sediment of a saline lake in southwest of Algeria
%D 2013
%L fdi:010060800
%G ENG
%J Current Microbiology
%@ 0343-8651
%K ALGERIE
%M ISI:000317080100007
%N 5
%P 462-466
%R 10.1007/s00284-012-0300-7
%U https://www.documentation.ird.fr/hor/fdi:010060800
%> https://www.documentation.ird.fr/intranet/publi/2013/05/010060800.pdf
%V 66
%W Horizon (IRD)
%X A novel, Gram-positive, moderately halophilic bacterium, oxidase- and catalase-positive designated FarD(T) was isolated from sediments of a saline lake located in Taghit, 93 km from Bechar, southwest of Algeria. Cells were rod-shaped, endospore forming, and motile. Growth occurred at 15-40 A degrees C (optimum, 35 A degrees C), pH 6.0-12.0 (optimum, 7.0) and in the presence of 1-20 % NaCl (optimum, 10 %). Strain FarD(T) used glucose, mannitol, melibiose, d-mannose, and 5 ketogluconate. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and three phospholipids; MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C-15:0, anteiso C-17:0, C-20:0, and anteiso C-19:0. The DNA G+C content was 42.1 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA gene sequence indicated that strain FarD(T) had as its closest relative Virgibacillus salinus (similarity of 96.3 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain FarD(T) is proposed as a novel species of the genus Virgibacillus within the order Clostridiales, for which the name V. natechei is proposed. The type strain is FarD(T) (=DSM 25609(T) = CCUG 62224(T)).
%$ 084