@article{fdi:010060763,
  title = {{A} novel semi-multiplex {PCR} assay for identification of tropical eels of genus {A}nguilla in {I}ndonesian waters},
  author = {{F}ahmi, {M}. {R}. and {S}olihin, {D}. {D}. and {S}oewardi, {K}. and {P}ouyaud, {L}aurent and {S}hao, {Z}. {J}. and {B}errebi, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} one-step semi-multiplex polymerase chain reaction ({PCR}) is proposed to distinguish seven species and subspecies of tropical eels, i.e., {A}nguilla celebesensis, {A}. interioris, {A}. nebulosa nebulosa, {A}. marmorata, {A}. borneensis, {A}. bicolor bicolor, and {A}. bicolor pacifica, known in {I}ndonesian waters. {S}even pairs of species-specific primers, including two forward and seven reverse sequences, were designed after alignment of published and new complete mitochondrial cytochrome b (1,140 bp) and 16{S} ribosomal {RNA} (r{RNA}) (1,120 bp) sequences. {A}ll species-specific primer pairs are included in one {PCR} mix, but only one pair of them can amplify a specific fragment from the template {DNA} that is analyzed. {T}he semi-multiplex {PCR} amplified a fragment of 230 bp for {A}. b. bicolor, 372 bp for {A}. n. nebulosa, 450 bp for {A}. borneensis, 620 bp for {A}. marmorata, 670 bp for {A}. b. pacifica, 720 bp for {A}. celebesensis, and 795 bp for {A}. interioris, which are then separated by {DNA} agarose gel electrophoresis.},
  keywords = {{S}emi-multiplex {PCR} ; {A}nguilla spp. ; {T}ropical eels ; {M}olecular identification ; {S}pecies-subspecies-specific primer ; {INDONESIE}},
  booktitle = {},
  journal = {{F}isheries {S}cience},
  volume = {79},
  numero = {2},
  pages = {185--191},
  ISSN = {0919-9268},
  year = {2013},
  DOI = {10.1007/s12562-012-0587-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060763},
}