%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Abbassi, F.
%A Raja, Z.
%A Oury, Bruno
%A Gazanion, Elodie
%A Piesse, C.
%A Sereno, Denis
%A Nicolas, P.
%A Foulon, T.
%A Ladram, A.
%T Antibacterial and leishmanicidal activities of temporin-SHd, a 17-residue long membrane-damaging peptide
%D 2013
%L fdi:010060735
%G ENG
%J Biochimie
%@ 0300-9084
%K Antimicrobial peptide ; Amphibian ; Temporin-SH ; Circular dichroism ; Membrane interaction/permeabilization ; Antiparasitic activity
%M ISI:000315614200031
%N 2
%P 388-399
%R 10.1016/j.biochi.2012.10.015
%U https://www.documentation.ird.fr/hor/fdi:010060735
%> https://www.documentation.ird.fr/intranet/publi/2013/04/010060735.pdf
%V 95
%W Horizon (IRD)
%X Temporins are a family of short antimicrobial peptides (8-17 residues) that mostly show potent activity against Gram-positive bacteria. Herein, we demonstrate that temporin-SHd, a 17-residue peptide with a net charge of +2 (FLPAALAGIGGILGKLF(amide)) expressed a broad spectrum of antimicrobial activity. This peptide displayed potent antibacterial activities against Gram-negative and Gram-positive bacteria, including multi-drug resistant Staphylococcus aureus strains, as well as antiparasitic activity against promastigote and the intracellular stage (amastigote) of Leishmania infantum, at concentration not toxic for the macrophages. Temporin-SHd that is structured in a non-amphipathic alpha-helix in anionic membrane-mimetic environments, strongly and selectively perturbs anionic bilayer membranes by interacting with the polar head groups and acyl region of the phospholipids, with formation of regions of two coexisting phases: one phase rich in peptide and the other lipid-rich. The disruption of lipid packing within the bilayer may lead to the formation of transient pores and membrane permeation/disruption once a threshold peptide accumulation is reached. To our knowledge, Temporin-SHd represents the first known 17-residue long temporin expressing such broad spectrum of antimicrobial activity including members of the trypanosomatidae family. Additionally, since only a few shorter members (13 residues) of the temporin family are known to display antileishmanial activity (temporins-TA, -TB and -SHa), SHd is an interesting tool to analyze the antiparasitic mechanism of action of temporins.
%$ 020 ; 052