@article{fdi:010060721,
  title = {{I}dentification of cryptic {A}nopheles mosquito species by molecular protein profiling},
  author = {{M}uller, {P}. and {P}fluger, {V}. and {W}ittwer, {M}. and {Z}iegler, {D}. and {C}handre, {F}abrice and {S}imard, {F}r{\'e}d{\'e}ric and {L}engeler, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{V}ector control is the mainstay of malaria control programmes. {S}uccessful vector control profoundly relies on accurate information on the target mosquito populations in order to choose the most appropriate intervention for a given mosquito species and to monitor its impact. {A}n impediment to identify mosquito species is the existence of morphologically identical sibling species that play different roles in the transmission of pathogens and parasites. {C}urrently {PCR} diagnostics are used to distinguish between sibling species. {PCR} based methods are, however, expensive, time-consuming and their development requires a priori {DNA} sequence information. {H}ere, we evaluated an inexpensive molecular proteomics approach for {A}nopheles species: matrix assisted laser desorption/ ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}). {MALDI}-{TOF} {MS} is a well developed protein profiling tool for the identification of microorganisms but so far has received little attention as a diagnostic tool in entomology. {W}e measured {MS} spectra from specimens of 32 laboratory colonies and 2 field populations representing 12 {A}nopheles species including the {A}. gambiae species complex. {A}n important step in the study was the advancement and implementation of a bioinformatics approach improving the resolution over previously applied cluster analysis. {B}orrowing tools for linear discriminant analysis from genomics, {MALDI}-{TOF} {MS} accurately identified taxonomically closely related mosquito species, including the separation between the {M} and {S} molecular forms of {A}. gambiae sensu stricto. {T}he approach also classifies specimens from different laboratory colonies; hence proving also very promising for its use in colony authentication as part of quality assurance in laboratory studies. {W}hile being exceptionally accurate and robust, {MALDI}-{TOF} {MS} has several advantages over other typing methods, including simple sample preparation and short processing time. {A}s the method does not require {DNA} sequence information, data can also be reviewed at any later stage for diagnostic or functional patterns without the need for re-designing and re-processing biological material.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {2},
  pages = {e57486},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0057486},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060721},
}