%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Markovic, Z.
%A Chatelet, P.
%A Sylvestre, Isabelle
%A Kontic, J. K.
%A Engelmann, Florent
%T Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips
%D 2013
%L fdi:010060609
%G ENG
%J Central European Journal of Biology
%@ 1895-104X
%K Encapsulation-dehydration ; Droplet-vitrification ; Shoot tip size
%M ISI:000322674300007
%N 10
%P 993-1000
%R 10.2478/s11535-013-0223-8
%U https://www.documentation.ird.fr/hor/fdi:010060609
%> https://www.documentation.ird.fr/intranet/publi/2013/09/010060609.pdf
%V 8
%W Horizon (IRD)
%X In this work, we compared the efficiency of encapsulation-dehydration and droplet-vitrification techniques for cryopreserving grapevine (Vitis vinifera L.) cv. Portan shoot tips. Recovery of cryopreserved samples was achieved with both techniques; however, droplet-vitrification, which was used for the first time with grapevine shoot tips, produced higher regrowth. With encapsulationdehydration, encapsulated shoot tips were precultured in liquid medium with progressively increasing sucrose concentrations over a 2-day period (12 h in medium with 0.25, 0.5, 0.75 and 1.0 M sucrose), then dehydrated to 22.28% moisture content (fresh weight). After liquid nitrogen exposure 37.1% regrowth was achieved using 1 mm-long shoot tips and only 16.0% with 2 mm-long shoot tips. With droplet-vitrification, 50% regrowth was obtained following treatment of shoot tips with a loading solution containing 2 M glycerol + 0.4 M sucrose for 20 min, dehydration with half-strength PVS2 vitrification solution (30% (w/v) glycerol, 15% (w/v) ethylene glycol, 15% dimethylsulfoxide and 0.4 M sucrose in basal medium) at room temperature, then with full strength PVS2 solution at 0A degrees C for 50 min before direct immersion in liquid nitrogen. No regrowth was achieved after cryopreservation when shoot tips were dehydrated with PVS3 vitrification solution (50% (w/v) glycerol and 50% (w/v) sucrose in basal medium).
%$ 076 ; 084