@article{fdi:010060609,
  title = {{C}ryopreservation of grapevine ({V}itis vinifera {L}.) in vitro shoot tips},
  author = {{M}arkovic, {Z}. and {C}hatelet, {P}. and {S}ylvestre, {I}sabelle and {K}ontic, {J}. {K}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this work, we compared the efficiency of encapsulation-dehydration and droplet-vitrification techniques for cryopreserving grapevine ({V}itis vinifera {L}.) cv. {P}ortan shoot tips. {R}ecovery of cryopreserved samples was achieved with both techniques; however, droplet-vitrification, which was used for the first time with grapevine shoot tips, produced higher regrowth. {W}ith encapsulationdehydration, encapsulated shoot tips were precultured in liquid medium with progressively increasing sucrose concentrations over a 2-day period (12 h in medium with 0.25, 0.5, 0.75 and 1.0 {M} sucrose), then dehydrated to 22.28% moisture content (fresh weight). {A}fter liquid nitrogen exposure 37.1% regrowth was achieved using 1 mm-long shoot tips and only 16.0% with 2 mm-long shoot tips. {W}ith droplet-vitrification, 50% regrowth was obtained following treatment of shoot tips with a loading solution containing 2 {M} glycerol + 0.4 {M} sucrose for 20 min, dehydration with half-strength {PVS}2 vitrification solution (30% (w/v) glycerol, 15% (w/v) ethylene glycol, 15% dimethylsulfoxide and 0.4 {M} sucrose in basal medium) at room temperature, then with full strength {PVS}2 solution at 0{A} degrees {C} for 50 min before direct immersion in liquid nitrogen. {N}o regrowth was achieved after cryopreservation when shoot tips were dehydrated with {PVS}3 vitrification solution (50% (w/v) glycerol and 50% (w/v) sucrose in basal medium).},
  keywords = {{E}ncapsulation-dehydration ; {D}roplet-vitrification ; {S}hoot tip size},
  booktitle = {},
  journal = {{C}entral {E}uropean {J}ournal of {B}iology},
  volume = {8},
  numero = {10},
  pages = {993--1000},
  ISSN = {1895-104{X}},
  year = {2013},
  DOI = {10.2478/s11535-013-0223-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060609},
}