@article{fdi:010060588,
  title = {{M}atrix-assisted laser desorption ionization - time of flight mass spectrometry : an emerging tool for the rapid identification of mosquito vectors},
  author = {{Y}ssouf, {A}. and {S}ocolovschi, {C}. and {F}laudrops, {C}. and {N}diath, {M}. {O}. and {S}ougoufara, {S}. and {D}ehecq, {J}. {S}. and {L}acour, {G}. and {B}erenger, {J}. {M}. and {S}okhna, {C}heikh and {R}aoult, {D}idier and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he identification of mosquito vectors is typically based on morphological characteristics using morphological keys of determination, which requires entomological expertise and training. {T}he use of protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF}-{MS}), which is increasingly being used for the routine identification of bacteria, has recently emerged for arthropod identification. {M}ethods: {T}o investigate the usefulness of {MALDI}-{TOF}-{MS} as a mosquito identification tool, we tested protein extracts made from mosquito legs to create a database of reference spectra. {T}he database included a total of 129 laboratory-reared and field-caught mosquito specimens consisting of 20 species, including 4 {A}edes spp., 9 {A}nopheles spp., 4 {C}ulex spp., {L}utzia tigripes, {O}rthopodomyia reunionensis and {M}ansonia uniformis. {F}or the validation study, blind tests were performed with 76 specimens consisting of 1 to 4 individuals per species. {A} cluster analysis was carried out using the {MALDI}-{B}iotyper and some spectra from all mosquito species tested. {R}esults: {B}iomarker mass sets containing 22 and 43 masses have been detected from 100 specimens of the {A}nopheles, {A}edes and {C}ulex species. {B}y carrying out 3 blind tests, we achieved the identification of mosquito vectors at the species level, including the differentiation of {A}n. gambiae complex, which is possible using {MALDI}-{TOF}-{MS} with 1.8 as the cut-off identification score. {A} cluster analysis performed with all available mosquito species showed that {MALDI}-{B}iotyper can distinguish between specimens at the subspecies level, as demonstrated for {A}n gambiae {M} and {S}, but this method cannot yet be considered a reliable tool for the phylogenetic study of mosquito species. {C}onclusions: {W}e confirmed that even without any specific expertise, {MALDI}-{TOF}-{MS} profiling of mosquito leg protein extracts can be used for the rapid identification of mosquito vectors. {T}herefore, {MALDI}-{TOF}-{MS} is an alternative, efficient and inexpensive tool that can accurately identify mosquitoes collected in the field during entomological surveys.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {8},
  pages = {e72380},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0072380},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060588},
}