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Yssouf A., Socolovschi C., Flaudrops C., Ndiath M. O., Sougoufara S., Dehecq J. S., Lacour G., Berenger J. M., Sokhna Cheikh, Raoult Didier, Parola P. (2013). Matrix-assisted laser desorption ionization - time of flight mass spectrometry : an emerging tool for the rapid identification of mosquito vectors. Plos One, 8 (8), e72380. ISSN 1932-6203

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Lien direct chez l'éditeur doi:10.1371/journal.pone.0072380

Titre
Matrix-assisted laser desorption ionization - time of flight mass spectrometry : an emerging tool for the rapid identification of mosquito vectors
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000323378000096
AuteursYssouf A., Socolovschi C., Flaudrops C., Ndiath M. O., Sougoufara S., Dehecq J. S., Lacour G., Berenger J. M., Sokhna Cheikh, Raoult Didier, Parola P.
SourcePlos One, 2013, 8 (8), p. e72380. p. e72380 ISSN 1932-6203
RésuméBackground: The identification of mosquito vectors is typically based on morphological characteristics using morphological keys of determination, which requires entomological expertise and training. The use of protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which is increasingly being used for the routine identification of bacteria, has recently emerged for arthropod identification. Methods: To investigate the usefulness of MALDI-TOF-MS as a mosquito identification tool, we tested protein extracts made from mosquito legs to create a database of reference spectra. The database included a total of 129 laboratory-reared and field-caught mosquito specimens consisting of 20 species, including 4 Aedes spp., 9 Anopheles spp., 4 Culex spp., Lutzia tigripes, Orthopodomyia reunionensis and Mansonia uniformis. For the validation study, blind tests were performed with 76 specimens consisting of 1 to 4 individuals per species. A cluster analysis was carried out using the MALDI-Biotyper and some spectra from all mosquito species tested. Results: Biomarker mass sets containing 22 and 43 masses have been detected from 100 specimens of the Anopheles, Aedes and Culex species. By carrying out 3 blind tests, we achieved the identification of mosquito vectors at the species level, including the differentiation of An. gambiae complex, which is possible using MALDI-TOF-MS with 1.8 as the cut-off identification score. A cluster analysis performed with all available mosquito species showed that MALDI-Biotyper can distinguish between specimens at the subspecies level, as demonstrated for An gambiae M and S, but this method cannot yet be considered a reliable tool for the phylogenetic study of mosquito species. Conclusions: We confirmed that even without any specific expertise, MALDI-TOF-MS profiling of mosquito leg protein extracts can be used for the rapid identification of mosquito vectors. Therefore, MALDI-TOF-MS is an alternative, efficient and inexpensive tool that can accurately identify mosquitoes collected in the field during entomological surveys.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
LocalisationFonds IRD [F B010060588]
Identifiant IRDfdi:010060588
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010060588

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