Garzon E., Holzmuller P., Bras Goncalves Rachel, Vincendeau P., Cuny Gérard, Lemesre Jean-Loup, Geiger Anne. (2013). The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine production, and allostimulatory capacity of dendritic cells. Infection and Immunity, 81 (9), p. 3300-3308. ISSN 0019-9567.
Titre du document
The Trypanosoma brucei gambiense secretome impairs lipopolysaccharide-induced maturation, cytokine production, and allostimulatory capacity of dendritic cells
Année de publication
2013
Auteurs
Garzon E., Holzmuller P., Bras Goncalves Rachel, Vincendeau P., Cuny Gérard, Lemesre Jean-Loup, Geiger Anne
Source
Infection and Immunity, 2013,
81 (9), p. 3300-3308 ISSN 0019-9567
Trypanosoma brucei gambiense, a parasitic protozoan belonging to kinetoplastids, is the main etiological agent of human African trypanosomiasis (HAT), or sleeping sickness. One major characteristic of this disease is the dysregulation of the host immune system. The present study demonstrates that the secretome (excreted-secreted proteins) of T. b. gambiense impairs the lipopolysaccharide (LPS)-induced maturation of murine dendritic cells (DCs). The upregulation of major histocompatibility complex class II, CD40, CD80, and CD86 molecules, as well as the secretion of cytokines such as tumor necrosis factor alpha, interleukin-10 (IL-10), and IL-6, which are normally released at high levels by LPS-stimulated DCs, is significantly reduced when these cells are cultured in the presence of the T. b. gambiense secretome. Moreover, the inhibition of DC maturation results in the loss of their allostimulatory capacity, leading to a dramatic decrease in Th1/Th2 cytokine production by cocultured lymphocytes. These results provide new insights into a novel efficient immunosuppressive mechanism directly involving the alteration of DC function which might be used by T. b. gambiense to interfere with the host immune responses in HAT and promote the infectious process.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
AFRIQUE SUBSAHARIENNE
Localisation
Fonds IRD [F B010060586]
Identifiant IRD
fdi:010060586
