@article{fdi:010060565,
  title = {{I}solation of infectious chikungunya virus and dengue virus using anionic polymer-coated magnetic beads},
  author = {{P}atramool, {S}. and {B}ernard, {E}. and {H}amel, {R}odolphe and {N}atthanej, {L}. and {C}hazal, {N}. and {S}urasombatpattana, {P}ornapat and {E}kchariyawat, {P}. and {D}aoust, {S}. and {T}hongrungkiat, {S}. and {T}homas, {F}. and {B}riant, {L}. and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{M}osquitoes-borne viruses are a major threat for human populations. {A}mong them, chikungunya virus ({CHIKV}) and dengue virus ({DENV}) cause thousands of cases worldwide. {T}he recent propagation of mosquito vectors competent to transmit these viruses to temperate areas increases their potential impact on susceptible human populations. {T}he development of sensitive methods allowing the detection and isolation of infectious viruses is of crucial interest for determination of virus contamination in humans and in competent mosquito vectors. {H}owever, simple and rapid method allowing the capture of infectious {CHIKV} and {DENV} from samples with low viral titers useful for further genetic and functional characterization of circulating strains is lacking. {T}he present study reports a fast and sensitive isolation technique based on viral particles adsorption on magnetic beads coated with anionic polymer, poly(methyl vinyl ether-maleic anhydrate) and suitable for isolation of infectious {CHIKV} and {DENV} from the four serotypes. {S}tarting from quite reduced biological material, this method was accurate to combine with conventional detection techniques, including q{RT}-{PCR} and immunoblotting and allowed isolation of infectious particles without resorting to a step of cultivation. {T}he use of polymer-coated magnetic beads is therefore of high interest for rapid detection and isolation of {CHIKV} and {DENV} from samples with reduced viral loads and represents an accurate approach for the surveillance of mosquito vector in area at risk for arbovirus outbreaks.},
  keywords = {{A}rbovirus ; {C}hikungunya ; {D}engue ; {M}agnetic beads ; {V}iro-{A}dembeads},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {193},
  numero = {1},
  pages = {55--61},
  ISSN = {0166-0934},
  year = {2013},
  DOI = {10.1016/j.jviromet.2013.04.016},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060565},
}