@article{fdi:010060561,
  title = {{G}enotyping and antifungal susceptibility testing of {C}ryptococcus neoformans isolates from {C}ameroonian {HIV}-positive adult patients},
  author = {{B}ertout, {S}. and {D}rakulovski, {P}. and {K}ouanfack, {C}. and {K}rasteva, {D}. and {N}gouana, {T}. and {D}unyach-{R}emy, {C}. and {D}ongtsa, {J}. and {A}ghokeng {F}obang, {A}velin and {D}elaporte, {E}ric and {K}oulla-{S}hiro, {S}. and {R}eynes, {J}. and {M}allie, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ryptococcus neoformans is the most common cause of meningitis amongst adult {A}fricans with {HIV}/{AIDS}. {T}he widespread use of fluconazole may lead to the emergence of isolates with reduced susceptibility. {W}e studied {C}.neoformans isolates from {HIV}-infected patients with cryptococcal meningitis. {G}enotyping and antifungal testing were performed to assess the genetic diversity, occurrence of mixed infections and in vitro activity of antifungal agents. {I}solates were recovered from cerebrospinal fluid prior to systemic antifungal treatment. {S}ix isolates were studied for each sample (a total of 114 isolates from 19 patients). {S}erotyping was performed via {LAC} 1 and {CAP} 64 gene amplification and genotyping was performed using phage {M}13 core, ({GACA})(4) and ({GTG})(5) primers and restriction polymorphism analysis of the {URA}5 gene. {S}usceptibilities for amphotericin {B}, flucytosine, fluconazole, voriconazole and posaconazole were tested by the {S}ensititre {Y}east{O}ne ({R}) method. {A}ll strains were identified as {C}.neoformans var. grubii serotype {A}. {W}e identified nine major genotypes. {U}p to two genotypes were identified in the same sample. {N}one of the isolates were resistant to the studied drugs. {H}owever, 13 of 114 strains exhibited a reduced susceptibility to fluconazole and 13 of 114 strains exhibited a reduced susceptibility to flucytosine. {N}o correlation was found between the genotype and susceptibility. {T}his study confirms the prevalence of {C}.neoformans serotype {A} in {C}ameroon. {T}wo genotypes may be responsible for a single episode of cryptococcosis. {T}he possibility of mixed infection and diminished susceptibility to fluconazole or flucytosine must be considered for the management of cryptococcosis.},
  keywords = {{AIDS} ; antifungal ; cryptococcosis ; genetic diversity ; genotyping ; {CAMEROUN}},
  booktitle = {},
  journal = {{C}linical {M}icrobiology and {I}nfection},
  volume = {19},
  numero = {8},
  pages = {763--769},
  ISSN = {1198-743{X}},
  year = {2013},
  DOI = {10.1111/1469-0691.12019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060561},
}