@article{fdi:010060548,
  title = {{S}ilencing of the chalcone synthase gene in {C}asuarina glauca highlights the important role of flavonoids during nodulation},
  author = {{A}bdel-{L}ateif, {K}. and {V}aissayre, {V}irginie and {G}herbi, {H}assen and {V}erries, {C}. and {M}eudec, {E}. and {P}errine-{W}alker, {F}. and {C}heynier, {V}. and {S}vistoonoff, {S}ergio and {F}ranche, {C}laudine and {B}ogusz, {D}idier and {H}ocher, {V}al{\'e}rie},
  editor = {},
  language = {{ENG}},
  abstract = {{N}itrogen-fixing root nodulation is confined to four plant orders, including > 14 000 {L}eguminosae, one nonlegume genus {P}arasponia and c. 200 actinorhizal species that form symbioses with rhizobia and {F}rankia bacterial species, respectively. {F}lavonoids have been identified as plant signals and developmental regulators for nodulation in legumes and have long been hypothesized to play a critical role during actinorhizal nodulation. {H}owever, direct evidence of their involvement in actinorhizal symbiosis is lacking. {H}ere, we used {RNA} interference to silence chalcone synthase, which is involved in the first committed step of the flavonoid biosynthetic pathway, in the actinorhizal tropical tree {C}asuarina glauca. {T}ransformed flavonoid-deficient hairy roots were generated and used to study flavonoid accumulation and further nodulation. {K}nockdown of chalcone synthase expression reduced the level of specific flavonoids and resulted in severely impaired nodulation. {N}odule formation was rescued by supplementing the plants with naringenin, which is an upstream intermediate in flavonoid biosynthesis. {O}ur results provide, for the first time, direct evidence of an important role for flavonoids during the early stages of actinorhizal nodulation.},
  keywords = {actinorhizal symbiosis ; {C}asuarina glauca ; chalcone synthase ; endosymbiosis ; flavonoids ; {F}rankia ; {RNA} interference ; signaling},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {199},
  numero = {4},
  pages = {1012--1021},
  ISSN = {1469-8137},
  year = {2013},
  DOI = {10.1111/nph.12326},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060548},
}