@article{fdi:010060533,
  title = {{U}se of colorimetric culture methods for detection of {M}ycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings},
  author = {{B}oum, {Y}. and {O}rikiriza, {P}. and {R}ojas-{P}once, {G}. and {R}iera-{M}ontes, {M}. and {A}twine, {D}. and {N}ansumba, {M}. and {B}azira, {J}. and {T}uyakira, {E}. and {B}eaudrap, {P}ierre de and {B}onnet, {M}. and {P}age, {A}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}espite recent advances, tuberculosis ({TB}) diagnosis remains imperfect in resource-limited settings due to its complexity and costs, poor sensitivity of available tests, or long times to reporting. {W}e present a report on the use of colorimetric methods, based on the detection of mycobacterial growth using colorimetric indicators, for the detection of {M}ycobacterium tuberculosis in sputum specimens. {W}e evaluated the nitrate reductase assay ({NRA}), a modified {NRA} using para-nitrobenzoic acid ({PNB}) ({NRA}p), and the resazurin tube assay using {PNB} ({RETA}p) to differentiate tuberculous and nontuberculous mycobacteria. {T}he performances were assessed at days 18 and 28 using mycobacterium growth indicator tube ({MGIT}) and {L}owenstein-{J}ensen ({LJ}) medium culture methods as the reference standards. {W}e enrolled 690 adults with suspected pulmonary tuberculosis from a regional referral hospital in {U}ganda between {M}arch 2010 and {J}une 2011. {A}t day 18, the sensitivities and specificities were 84.6% and 90.0% for the {NRA}, 84.1% and 92.6% for the {NRA}p, and 71.2% and 99.3% for the {RETA}p, respectively. {A}t day 28, the sensitivity of the {RETA}p increased to 82.6%. {A}mong smear-negative patients with suspected {TB}, sensitivities at day 28 were 64.7% for the {NRA}, 61.3% for the {NRA}p, and 50% for the {RETA}p. {C}ontamination rates were found to be 5.4% for the {NRA} and 6.7% for the {RETA}p, compared with 22.1% for {LJ} medium culture and 20.4% for {MGIT} culture. {T}he median times to positivity were 10, 7, and 25 days for colorimetric methods, {MGIT} culture, and {LJ} medium culture, respectively. {W}hereas the low specificity of the {NRA}/{NRA}p precludes it from being used for {TB} diagnosis, the {RETA}p might provide an alternative to {LJ} medium culture to decrease the time to culture results in resource-poor settings.},
  keywords = {{OUGANDA}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {51},
  numero = {7},
  pages = {2273--2279},
  ISSN = {0095-1137},
  year = {2013},
  DOI = {10.1128/jcm.00749-13},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060533},
}