@article{fdi:010060524, title = {{N}ew methods for field collection of human skin volatiles and perspectives for their application in the chemical ecology of human-pathogen-vector interactions}, author = {{D}ormont, {L}. and {B}essiere, {J}. {M}. and {M}c{K}ey, {D}. and {C}ohuet, {A}nna}, editor = {}, language = {{ENG}}, abstract = {{O}dours emitted by human skin are of great interest to biologists in many fields, with practical applications in forensics, health diagnostic tools and the ecology of blood-sucking insect vectors of human disease. {C}onvenient methods are required for sampling human skin volatiles under field conditions. {W}e experimentally compared four modern methods for sampling skin odours: solvent extraction, headspace solid-phase micro-extraction ({SPME}), and two new techniques not previously used for the study of mammal volatiles, contact {SPME} and dynamic headspace with a chromatoprobe design. {T}hese methods were tested and compared both on {E}uropean subjects under laboratory conditions and on young {A}frican subjects under field conditions. {A}ll four methods permitted effective trapping of skin odours, including the major known human skin volatile compounds. {I}n both laboratory and field experiments, contact {SPME}, in which the time of collection was restricted to 3 min, provided results very similar to those obtained with classical headspace {SPME}, a method that requires 45 min of collection. {C}hromatoprobe sampling also proved to be very sensitive, rapid and convenient for the collection of human-produced volatiles in natural settings. {B}oth contact {SPME} and chromatoprobe design may considerably facilitate the study of human skin volatiles under field conditions, opening new possibilities for examining the olfactory cues mediating the host-seeking behaviour of mosquito vectors implicated in the transmission of major diseases.}, keywords = {chromatoprobe ; human ; sampling method ; skin odour ; {SPME}}, booktitle = {}, journal = {{J}ournal of {E}xperimental {B}iology}, volume = {216}, numero = {15}, pages = {2783--2788}, ISSN = {0022-0949}, year = {2013}, DOI = {10.1242/jeb.085936}, URL = {https://www.documentation.ird.fr/hor/fdi:010060524}, }