%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Liégeois, Florian
%A Reteno, D. G. I.
%A Mouinga-Ondeme, A.
%A Sica, J.
%A Rouet, F.
%T High natural polymorphism in the gag gene cleavage sites of non-B HIV type 1 isolates from Gabon
%D 2013
%L fdi:010060512
%G ENG
%J Aids Research and Human Retroviruses
%@ 0889-2229
%K GABON
%M ISI:000321949400014
%N 8
%P 1179-1182
%R 10.1089/aid.2013.0024
%U https://www.documentation.ird.fr/hor/fdi:010060512
%> https://www.documentation.ird.fr/intranet/publi/2013/08/010060512.pdf
%V 29
%W Horizon (IRD)
%X The main goal of the present study was to determine the frequency of substitutions in the cleavage sites (CS) of gag gene among non-B HIV-1 isolates from Gabon. Fifty plasma specimens, collected in 2010-2011, from HIV-1-infected patients failing first-line antiretroviral (ARV) regimens (constituted of two nucleoside reverse transcriptase inhibitors+one nonnucleoside reverse transcriptase inhibitor) (n = 38) and from HIV-1-infected individuals untreated with ARV (n = 12) were analyzed in the gag and gag-pol cleavage sites. Compared to HXB2 reference sequence, the total median number of substitutions in gag and gag-pol CS was 10 (range, 5-18). The cleavage site p2/NC was the most variable of the four gag CS with 100% (50/50) isolates carrying at least 1 substitution (range, 1-9). The two gag-pol TFP/p6(pol) and p6(pol)/PR CS sites were also highly variable (at least one substitution, 50/50, 100% in both cases). Substitutions at position G381 (p2/NC), L449 (p1/p6(gag)), and K444 (TFP/p6(pol)) were significantly more frequent in CRF02_AG strains, compared to other non-B strains (30.4% vs. 3.7%, p = 0.03; 87.0% vs. 59.3%, p = 0.03; and 91.3% vs. 59.3%, p = 0.01, respectively). Other non-B subtypes were significantly more likely to harbor substitutions at position N487 (p6(pol)) (70.4%) than CRF02_AG (39.1%) (p = 0.02). In Gabon, gag and gag-pol cleavage sites were highly polymorphic in protease inhibitor-naive patients harboring non-B HIV-1 strains. In sub-Saharan Africa, further studies are definitively required to better understand the impact of gag mutations among subjects receiving second-line LPV/r-containing regimens (monotherapy or triple combinations).
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