@article{fdi:010060487,
  title = {{E}vidence for novel hepaciviruses in rodents},
  author = {{D}rexler, {J}. {F}. and {C}orman, {V}. {M}. and {M}uller, {M}. {A}. and {L}ukashev, {A}. {N}. and {G}myl, {A}. and {C}outard, {B}. and {A}dam, {A}. and {R}itz, {D}. and {L}eijten, {L}. {M}. and van {R}iel, {D}. and {K}allies, {R}. and {K}lose, {S}. {M}. and {G}loza-{R}ausch, {F}. and {B}inger, {T}. and {A}nnan, {A}. and {A}du-{S}arkodie, {Y}. and {O}ppong, {S}. and {B}ourgarel, {M}. and {R}upp, {D}. and {H}offmann, {B}. and {S}chlegel, {M}. and {K}ummerer, {B}. {M}. and {K}ruger, {D}. {H}. and {S}chmidt-{C}hanasit, {J}. and {S}etien, {A}. {A}. and {C}ottontail, {V}. {M}. and {H}emachudha, {T}. and {W}acharapluesadee, {S}. and {O}sterrieder, {K}. and {B}artenschlager, {R}. and {M}atthee, {S}. and {B}eer, {M}. and {K}uiken, {T}. and {R}eusken, {C}. and {L}eroy, {E}ric and {U}lrich, {R}. {G}. and {D}rosten, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}epatitis {C} virus ({HCV}) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. {R}esearch is complicated by a lack of accessible small animal models. {T}he systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. {W}e have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). {T}hree highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 {E}uropean bank voles ({M}yodes glareolus) and 10 (1.9%) of 518 {S}outh {A}frican four-striped mice ({R}habdomys pumilio). {B}ats showed anti-{HCV} immunoblot reactivities but no virus detection, although the genetic relatedness suggested by the serologic results should have enabled {RNA} detection using the broadly reactive {PCR} assays developed for this study. 210 horses and 858 cats and dogs were tested, yielding further horse-associated hepaciviruses but none in dogs or cats. {T}he rodent viruses were equidistant to {HCV}, exceeding by far the diversity of {HCV} and the canine/equine hepaciviruses taken together. {F}ive full genomes were sequenced, representing all viral lineages. {S}alient genome features and distance criteria supported classification of all viruses as hepaciviruses. {Q}uantitative {RT}-{PCR}, {RNA} in-situ hybridisation, and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis {C}. {R}ecombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%, respectively. {A}ntibodies in bank vole sera neither cross-reacted with {HCV}, nor the heterologous bank vole hepacivirus. {C}o-occurrence of {RNA} and antibodies was found in 3 of 57 {PCR}-positive bank vole sera (5.3%). {O}ur data enable new hypotheses regarding {HCV} evolution and encourage efforts to develop rodent surrogate models for {HCV}.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {P}athogens},
  volume = {9},
  numero = {6},
  pages = {e1003438},
  ISSN = {1553-7374},
  year = {2013},
  DOI = {10.1371/journal.ppat.1003438},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060487},
}