@article{fdi:010060443,
  title = {{C}omplex evolutionary pathways of the intergenic region of the mini-exon gene in {T}rypanosoma cruzi {T}c{I} : {A} possible ancient origin in the {G}ran {C}haco and lack of strict genetic structuration},
  author = {{H}errera, {C}. {P}. and {B}arnab{\'e}, {C}hristian and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {T}c{I} discrete typing unit ({DTU}) of {T}rypanosoma cruzi is the most abundant and widely spread in the {A}mericas. {I}t is found in a wide range of triatomine and mammal species, which are distributed throughout the {A}mericas in sylvatic and domestic environments. {P}revious studies based on intergenic sequences of the mini-exon gene ({SL}-{IR}) have identified five genotype groups within {T}c{I}. {B}ased in the large number of sequences available in {G}en{B}ank, the present study conducted an exhaustive revision of the sequence variability of the {SL}-{IR} within {T}c{I} using 244 sequences from isolates, cellular or molecular clones, from 11 {L}atin {A}merican countries. {F}irst, the evolutionary branching between strains was examined by analyzing only the single nucleotide polymorphism ({SNP}) deleting the microsatellite region and the gaps from the total alignment. {T}hen the variability of the microsatellite region was reanalyzed alone using principal component analysis ({PCA}). {A}fter haplotype reconstruction using the {PHASE} algorithm, because of the presence of several ambiguous nucleotides in the {SNP} region, a total of 131 different haplotypes were obtained. {T}he topology reveals how difficult it is to identify an obvious structure in {T}c{I} for most of the parameters examined. {S}omewhat genetic and geographical structures exist, but no structure was depicted with cycle and host origins. {I}ndeed, the long-lasting evolution with possible recombination events, the occurrence of several waves of geographical dispersions (old and recent), and the high flow of strains between sylvatic and domestic cycles partially hide the major evolutionary trends within {T}c{I}. {M}oreover, we identified several problems in previous analyses, and concluded that in absence of supplementary studies of {T}c{I} phylogeny with other genetic markers, it is hazardous to use only the mini-exon intergenic region as a relevant marker of the sub-structure within {T}c{I}.},
  keywords = {{T}rypanosoma cruzi ; {M}ini-exon gene ; {T}rypanosoma cruzi {I} ; {D}iscrete typing units ; {DTU} ; {R}eticulate evolution ; {AMERIQUE} {LATINE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {16},
  numero = {},
  pages = {27--37},
  ISSN = {1567-1348},
  year = {2013},
  DOI = {10.1016/j.meegid.2012.12.028},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060443},
}