%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Lokossou, A. G.
%A Dechavanne, Célia
%A Bouraima, A.
%A Courtin, David
%A Le Port, A.
%A Ladekpo, R.
%A Noukpo, J.
%A Bonou, D.
%A Ahouangninou, C.
%A Sabbagh, A.
%A Fayomi, B.
%A Massougbodji, A.
%A Garcia, André
%A Migot Nabias, Florence
%T Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns
%D 2013
%L fdi:010060417
%G ENG
%J Bmc Infectious Diseases
%@ 1471-2334
%K Malaria ; P. falciparum ; Cytokine gene polymorphisms ; IL-4 ; IL-10 ; IL-13 ; Pregnancy ; Cord blood ; Recombinant proteins ; Specific antibodies
%M ISI:000320052800001
%P 215
%R 10.1186/1471-2334-13-215
%U https://www.documentation.ird.fr/hor/fdi:010060417
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers15-11/010060417.pdf
%V 13
%W Horizon (IRD)
%X Background: Particular cytokine gene polymorphisms are involved in the regulation of the antibody production. The consequences of already described IL-4, IL-10 and IL-13 gene polymorphisms on biological parameters and antibody levels were investigated among 576 mothers at delivery and their newborns in the context of P. falciparum placental malaria infection. Methods: The study took place in the semi-rural area of Tori-Bossito, in south-west Benin, where malaria is meso-endemic. Six biallelic polymorphisms were determined by quantitative PCR using TaqMan (R) Pre Designed SNP Genotyping Assays, in IL-4 (rs2243250, rs2070874), IL-10 (rs1800896, rs1800871, rs1800872) and IL-13 (rs1800925) genes. Antibody responses directed to P. falciparum MSP-1, MSP-2, MSP-3, GLURP-R0, GLURP-R2 and AMA-1 recombinant proteins were determined by ELISA. Results: The maternal IL-4(-590)*T/IL-4(+33)*T haplotype (one or two copies) was associated with favorable maternal condition at delivery (high haemoglobin levels, absence of placental parasites) and one of its component, the IL-4(-590) TT genotype, was related to low IgG levels to MSP-1, MSP-2/3D7 and MSP-2/FC27. Inversely, the maternal IL-10(-1082) AA was positively associated with P. falciparum placenta infection at delivery. As a consequence, the IL-10(-819)*T allele (in CT and TT genotypes) as well as the IL-10(-1082)*A/IL-10(-819)*T/IL-10(-592)*A haplotype (one or two copies) in which it is included, were related to an increased risk for anaemia in newborns. The maternal IL-10(-1082) AA genotype was related to high IgG levels to MSP-2/3D7 and AMA-1 in mothers and newborns, respectively. The IL-13 gene polymorphism was only involved in the newborn's antibody response to AMA-1. Conclusion: These data revealed that IL-4 and IL-10 maternal gene polymorphisms are likely to play a role in the regulation of biological parameters in pregnant women at delivery (anaemia, P. falciparum placenta infection) and in newborns (anaemia). Moreover, IL-4, IL-10 and IL-13 maternal gene polymorphisms were related to IgG responses to MSP-1, MSP-2/3D7 and MSP-2/FC27 in mothers as well as to AMA-1 in newborns.
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