%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Auguy, Florence
%A Fahr, M.
%A Moulin Esmard, Patricia
%A Brugel, A.
%A Laplaze, Laurent
%A El Mzibri, M.
%A Filali-Maltouf, A.
%A Doumas, P.
%A Smouni, A.
%T Lead tolerance and accumulation in Hirschfeldia incana, a Mediterranean Brassicaceae from metalliferous mine spoils
%D 2013
%L fdi:010060401
%G ENG
%J Plos One
%@ 1932-6203
%K MAROC
%M ISI:000319654700018
%N 5
%P art. e61932
%R 10.1371/journal.pone.0061932
%U https://www.documentation.ird.fr/hor/fdi:010060401
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010060401.pdf
%V 8
%W Horizon (IRD)
%X Lead is a heavy metal of particular concern with respect to environmental quality and health. The lack of plant species that accumulate and tolerate Pb is a limiting factor to understand the molecular mechanisms involved in Pb tolerance. In this study we identified Hirschfeldia incana, a Brassicaceae collected from metalliferous mine spoils in Morocco, as a Pb accumulator plant. H. incana exhibited high Pb accumulation in mine soils and in hydroponic cultures. Major Pb accumulation occurred in the roots and a part of Pb translocated from the roots to the shoots, even to the siliques. These findings demonstrated that H. incana is a Pb accumulator species. The expression of several candidate genes after Pb-exposure was measured by quantitative PCR and two of them, HiHMA4 and HiMT2a, coding respectively for a P1B-type ATPase and a metallothionein, were particularly induced by Pb-exposure in both roots and leaves. The functional characterization of HiHMA4 and HiMT2a was achieved using Arabidopsis T-DNA insertional mutants. Pb content and primary root growth analysis confirmed the role of these two genes in Pb tolerance and accumulation. H. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants.
%$ 076 ; 084