@article{fdi:010060401, title = {{L}ead tolerance and accumulation in {H}irschfeldia incana, a {M}editerranean {B}rassicaceae from metalliferous mine spoils}, author = {{A}uguy, {F}lorence and {F}ahr, {M}. and {M}oulin {E}smard, {P}atricia and {B}rugel, {A}. and {L}aplaze, {L}aurent and {E}l {M}zibri, {M}. and {F}ilali-{M}altouf, {A}. and {D}oumas, {P}. and {S}mouni, {A}.}, editor = {}, language = {{ENG}}, abstract = {{L}ead is a heavy metal of particular concern with respect to environmental quality and health. {T}he lack of plant species that accumulate and tolerate {P}b is a limiting factor to understand the molecular mechanisms involved in {P}b tolerance. {I}n this study we identified {H}irschfeldia incana, a {B}rassicaceae collected from metalliferous mine spoils in {M}orocco, as a {P}b accumulator plant. {H}. incana exhibited high {P}b accumulation in mine soils and in hydroponic cultures. {M}ajor {P}b accumulation occurred in the roots and a part of {P}b translocated from the roots to the shoots, even to the siliques. {T}hese findings demonstrated that {H}. incana is a {P}b accumulator species. {T}he expression of several candidate genes after {P}b-exposure was measured by quantitative {PCR} and two of them, {H}i{HMA}4 and {H}i{MT}2a, coding respectively for a {P}1{B}-type {ATP}ase and a metallothionein, were particularly induced by {P}b-exposure in both roots and leaves. {T}he functional characterization of {H}i{HMA}4 and {H}i{MT}2a was achieved using {A}rabidopsis {T}-{DNA} insertional mutants. {P}b content and primary root growth analysis confirmed the role of these two genes in {P}b tolerance and accumulation. {H}. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants.}, keywords = {{MAROC}}, booktitle = {}, journal = {{P}los {O}ne}, volume = {8}, numero = {5}, pages = {art. e61932}, ISSN = {1932-6203}, year = {2013}, DOI = {10.1371/journal.pone.0061932}, URL = {https://www.documentation.ird.fr/hor/fdi:010060401}, }