Auguy Florence, Fahr M., Moulin Esmard Patricia, Brugel A., Laplaze Laurent, El Mzibri M., Filali-Maltouf A., Doumas P., Smouni A. (2013). Lead tolerance and accumulation in Hirschfeldia incana, a Mediterranean Brassicaceae from metalliferous mine spoils. Plos One, 8 (5), art. e61932. ISSN 1932-6203.
Titre du document
Lead tolerance and accumulation in Hirschfeldia incana, a Mediterranean Brassicaceae from metalliferous mine spoils
Auguy Florence, Fahr M., Moulin Esmard Patricia, Brugel A., Laplaze Laurent, El Mzibri M., Filali-Maltouf A., Doumas P., Smouni A.
Source
Plos One, 2013,
8 (5), art. e61932 ISSN 1932-6203
Lead is a heavy metal of particular concern with respect to environmental quality and health. The lack of plant species that accumulate and tolerate Pb is a limiting factor to understand the molecular mechanisms involved in Pb tolerance. In this study we identified Hirschfeldia incana, a Brassicaceae collected from metalliferous mine spoils in Morocco, as a Pb accumulator plant. H. incana exhibited high Pb accumulation in mine soils and in hydroponic cultures. Major Pb accumulation occurred in the roots and a part of Pb translocated from the roots to the shoots, even to the siliques. These findings demonstrated that H. incana is a Pb accumulator species. The expression of several candidate genes after Pb-exposure was measured by quantitative PCR and two of them, HiHMA4 and HiMT2a, coding respectively for a P1B-type ATPase and a metallothionein, were particularly induced by Pb-exposure in both roots and leaves. The functional characterization of HiHMA4 and HiMT2a was achieved using Arabidopsis T-DNA insertional mutants. Pb content and primary root growth analysis confirmed the role of these two genes in Pb tolerance and accumulation. H. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
