@article{fdi:010060391,
  title = {{B}ehavior of {L}actobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats},
  author = {{T}urpin, {W}illiams and {H}umblot, {C}hrist{\`e}le and {N}oordine, {M}. {L}. and {W}rzosek, {L}. and {T}omas, {J}. and {M}ayeur, {C}. and {C}herbuy, {C}. and {G}uyot, {J}ean-{P}ierre and {T}homas, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ost bacterial strains, which have been studied so far for their probiotic functions, are extensively used by manufacturers in developed countries. {I}n our work, we sought to study a mix (called {BSL}) comprising three strains belonging to {L}actobacillus fermentum, {L}. paraplantarum and {L}. salivarius, that were isolated from a traditional {A}frican pearl millet based fermented slurry. {O}ur objective was to study this {BSL} cocktail in gnotobiotic rats, to evaluate their survival and their behavior in the digestive tract conditions. {A}fter a single oral inoculation of germfree rats with {BSL}, the species established stably in the digestive tract with the following hierarchy of abundance: {L}. salivarius> {L}. plantarum> {L}. fermentum. {BSL} cocktail was metabolically active since it produced 50 m{M} lactate and it expressed genes involved in binding mechanism in the caecum. {F}urthermore, the global morphology of the colon epithelium was not disturbed by the {BSL} cocktail. {BSL} cocktail did not modify mucus content and host mucus-related genes ({MUC}1, {MUC}2, {MUC}3 or resistin-like molecule beta). {T}he cocktail of lactobacilli enhanced the proliferating cell nuclear antigen ({PCNA}) at a level comparable to what was observed in conventional rats. {PCNA} was involved in proliferation and {DNA} repair, but the presence of the cocktail did not provoke proliferative events (with {K}i67 as indicator), so we suppose {BSL} may help gut preservation. {T}his work is the first step towards the selection of strains that are derived from traditional fermented food to formulate new probiotic mixture.},
  keywords = {{AFRIQUE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {4},
  pages = {art. e57711},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0057711},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060391},
}