Dama E., Cornélie Sylvie, Somda M. B., Camara M., Kambire R., Courtin Fabrice, Jamonneau Vincent, Demettre E., Seveno M., Bengaly Z., Solano Philippe, Poinsignon Anne, Remoué Franck, Belem A. M. G., Bucheton Bruno. (2013). Identification of Glossina palpalis gambiensis specific salivary antigens : towards the development of a serologic biomarker of human exposure to tsetse flies in West Africa. Microbes and Infection, 15 (5), p. 416-427. ISSN 1286-4579.
Titre du document
Identification of Glossina palpalis gambiensis specific salivary antigens : towards the development of a serologic biomarker of human exposure to tsetse flies in West Africa
Année de publication
2013
Auteurs
Dama E., Cornélie Sylvie, Somda M. B., Camara M., Kambire R., Courtin Fabrice, Jamonneau Vincent, Demettre E., Seveno M., Bengaly Z., Solano Philippe, Poinsignon Anne, Remoué Franck, Belem A. M. G., Bucheton Bruno
Source
Microbes and Infection, 2013,
15 (5), p. 416-427 ISSN 1286-4579
The saliva of blood sucking arthropods contains a number of pharmacologically active compounds that induce an antibody response in exposed human individuals. The objectives of the present study were (i) to assess the human IgG response directed against salivary antigens of Glossina palpalis gambiensis, the main vector of Trypanosoma brucei gambiense in West Africa, as a biomarker of human tsetse contacts; and (ii) to identify specific salivary antigens. Immune reactivity of human plasma collected within active human African trypanosomiasis (HAT) foci (coastal Guinea), historical foci where tsetse flies are still present (South-West Burkina Faso) and a tsetse free area (Bobo-Dioulasso, Burkina Faso), was measured by ELISA against whole saliva extracts. In the active HAT foci and areas where tsetse flies were present in high densities, specific IgG responses were significantly higher (p < 0.0001) to those in Bobo-Dioulasso or in Loropeni, where tsetse flies were either absent or only present at low densities. Furthermore, 2D-electrophoresis combined with mass spectrometry enabled to reveal that several antigens were specifically recognized by plasma from exposed individuals. Among them, four salivary proteins were successfully identified (Ada, 5'Nuc, Ag5 and Tsgf1). These results represent a first attempt to identify Glossina salivary proteins or synthetic peptides to develop a standardized and specific biomarker of tsetse exposure in West Africa.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
AFRIQUE DE L'OUEST
Localisation
Fonds IRD [F B010060384]
Identifiant IRD
fdi:010060384
