@article{fdi:010060354,
  title = {{C}omparative analysis of rodent tissue preservation methods and nucleic acid extraction techniques for virus screening purposes},
  author = {{Y}ama, {I}. {N}. and {G}arba, {M}. and {B}ritton-{D}avidian, {J}. and {T}hiberville, {S}. {D}. and {D}obigny, {G}authier and {G}ould, {E}. {A}. and de {L}amballerie, {X}avier and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he polymerase chain reaction ({PCR}) has become an essential method for the detection of viruses in tissue specimens. {H}owever, it is well known that the presence of {PCR} inhibitors in tissue samples may cause false-negative results. {H}ence the identification of {PCR} inhibitors and evaluation and optimization of nucleic acid extraction and preservation methods is of prime concern in virus discovery programs dealing with animal tissues. {A}ccordingly, to monitor and remove inhibitors we have performed comparative analyses of two commonly used tissue storage methods and five {RNA} purification techniques using a variety of animal tissues, containing quantified levels of added {MS}2 bacteriophages as the indicator of inhibition. {T}he results showed (i) no significant difference between the two methods of sample preservation, viz. direct storage at -80 degrees {C} or 4 degrees {C} in {RNA}later, (ii) lung rodent tissues contained lower levels of inhibitor than liver, kidney and spleen, (iii) {RNA} extraction using the {EZ}1 + {PK} {RNA} kit was the most effective procedure for removal of {RT}-{PCR} inhibitors.},
  keywords = {{V}irus screening ; {PCR} inhibitors ; {O}rgan storage ; {P}roteinase {K} ; {E}nterobacteria phage {MS}2},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {189},
  numero = {2},
  pages = {311--316},
  ISSN = {0166-0934},
  year = {2013},
  DOI = {10.1016/j.jviromet.2013.01.024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060354},
}