@article{fdi:010060288,
  title = {{C}ryopreservation of {A}rachis pintoi ({L}eguminosae) somatic embryos},
  author = {{R}ey, {H}.{Y}. and {F}aloci, {M}. and {M}edina, {R}. and {D}olce, {N}. and {E}ngelmann, {F}lorent and {M}roginski, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid {A}rachis pintoi cytotypes using the encapsulation-dehydration technique. {T}he highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium ({EM}) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 {M}). {T}he encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, {LN}) or slowly (1º{C} min-1 from 25º{C} to -30º{C} followed by immersion in {LN}). {B}eads were kept in {LN} for a minimum of 1 h and then were rapidly rewarmed in a 30º{C} water-bath for 2 min. {F}inally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid {EM} with daily decreasing sucrose concentration (0.75 and 0.5 {M}) and transferred to solidified {EM}. {U}sing this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. {N}o morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine {RAPD} profiles.},
  keywords = {{LEGUMINEUSE} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {EMBRYOGENESE} {SOMATIQUE} ; {DESHYDRATATION} ; {EXPERIMENTATION} {IN} {VITRO} ; {ISOENZYME} ; {ANALYSE} {STATISTIQUE} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {CRYOPRESERVATION} ; {ENCAPSULATION} ; {SUCROSE} ; {STABILITE} {GENETIQUE} ; {TECHNIQUE} {RAPD}},
  booktitle = {},
  journal = {{C}ryo{L}etters},
  volume = {34},
  numero = {6},
  pages = {571--582},
  ISSN = {0143-2044},
  year = {2013},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060288},
}