%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sassi, A.
%A Darias, Maria Jose
%A Said, K.
%A Messaoudi, I.
%A Gisbert, E.
%T Cadmium exposure affects the expression of genes involved in skeletogenesis and stress response in gilthead sea bream larvae
%D 2013
%L fdi:010060279
%G ENG
%J Fish Physiology and Biochemistry
%@ 0920-1742
%K POISSON MARIN ; LARVE ; CONTAMINATION ; METAL LOURD ; METABOLISME ; GENE ; ARN ; OS ; MINERALISATION
%K DORADE ; CADMIUM ; EXPRESSION DES GENES ; TOLERANCE AU STRESS
%M WOS:000318731300019
%N 3
%P 649-659
%R 10.1007/s10695-012-9727-9
%U https://www.documentation.ird.fr/hor/fdi:010060279
%> https://www.documentation.ird.fr/intranet/publi/depot/2014-01-21/010060279.pdf
%V 39
%W Horizon (IRD)
%X Gilthead sea bream larvae (Sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of Cd2+ (0.1, 5 and 10 mg/L) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (metallothionein-mt, glutathione peroxidase 1-gpx1), stress response (heat shock protein 70-hsp70, tumour necrosis factor alpha-tnf alpha) and ossification (osteocalcin-oc) processes. For this purpose, specimens of 47 dph were exposed first for 72 h from 0.1 to 20 mg/L of Cd2+ in order to evaluate the median lethal concentration (LC50) for this metal, which was determined at 15.32 mg/L. Considering the results regarding the relative transcript levels of gpx1 and hsp70, Cd2+ at any of the tested levels (0.1, 5 and 10 mg/L) did not induce oxidative stress in gilthead sea bream larvae, whereas relative transcript levels of mt were increased at 5 and 10 mg/L of Cd2+ probably to detoxify this metal excess. Relative transcript levels of tnf alpha were not level dependent and were down-regulated in larvae exposed to 5 and 10 mg/L of Cd2+. At those concentrations, transcript levels of oc were down-regulated suggesting a disruption in bone mineralization. Results from this study provided insights in some molecular mechanisms underlying Cd2+-induced toxicity in fish at early stages of development. This is the first study to show that cadmium contamination can depress oc expression in teleosts.
%$ 034BIOVER01