@article{fdi:010060279,
  title = {{C}admium exposure affects the expression of genes involved in skeletogenesis and stress response in gilthead sea bream larvae},
  author = {{S}assi, {A}. and {D}arias, {M}aria {J}ose and {S}aid, {K}. and {M}essaoudi, {I}. and {G}isbert, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}ilthead sea bream larvae ({S}parus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of {C}d2+ (0.1, 5 and 10 mg/{L}) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (metallothionein-mt, glutathione peroxidase 1-gpx1), stress response (heat shock protein 70-hsp70, tumour necrosis factor alpha-tnf alpha) and ossification (osteocalcin-oc) processes. {F}or this purpose, specimens of 47 dph were exposed first for 72 h from 0.1 to 20 mg/{L} of {C}d2+ in order to evaluate the median lethal concentration ({LC}50) for this metal, which was determined at 15.32 mg/{L}. {C}onsidering the results regarding the relative transcript levels of gpx1 and hsp70, {C}d2+ at any of the tested levels (0.1, 5 and 10 mg/{L}) did not induce oxidative stress in gilthead sea bream larvae, whereas relative transcript levels of mt were increased at 5 and 10 mg/{L} of {C}d2+ probably to detoxify this metal excess. {R}elative transcript levels of tnf alpha were not level dependent and were down-regulated in larvae exposed to 5 and 10 mg/{L} of {C}d2+. {A}t those concentrations, transcript levels of oc were down-regulated suggesting a disruption in bone mineralization. {R}esults from this study provided insights in some molecular mechanisms underlying {C}d2+-induced toxicity in fish at early stages of development. {T}his is the first study to show that cadmium contamination can depress oc expression in teleosts.},
  keywords = {{POISSON} {MARIN} ; {LARVE} ; {CONTAMINATION} ; {METAL} {LOURD} ; {METABOLISME} ; {GENE} ; {ARN} ; {OS} ; {MINERALISATION} ; {DORADE} ; {CADMIUM} ; {EXPRESSION} {DES} {GENES} ; {TOLERANCE} {AU} {STRESS}},
  booktitle = {},
  journal = {{F}ish {P}hysiology and {B}iochemistry},
  volume = {39},
  numero = {3},
  pages = {649--659},
  ISSN = {0920-1742},
  year = {2013},
  DOI = {10.1007/s10695-012-9727-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060279},
}