@article{fdi:010058994,
  title = {{V}ariations in genomic {DNA} methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures},
  author = {{R}ival, {A}. and {I}lbert, {P}. and {L}abeyrie, {A}. and {T}orres, {E}. and {D}oulbeau, {S}ylvie and {P}ersonne, {A}. and {D}ussert, {S}t{\'e}phane and {B}eul{\'e}, {T}. and {D}urand-{G}asselin, {T}. and {T}regear, {J}ames and {J}aligot, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he long-term proliferation of embryogenic cell suspensions of oil palm is associated with changes in both genomic methylation rates and embryogenic capacities. {I}n the aim of exploring the relationship between epigenetic stability and the long-term in vitro proliferation of plant tissues, we have studied changes in genomic {DNA} methylation levels in embryogenic suspensions of oil palm ({E}laeis guineensis {J}acq.). {F}ive embryogenic callus lines were obtained from selected hybrid seeds and then proliferated as suspension cultures. {E}ach clonal line obtained from a single genotype was subdivided into three independent subclonal lines. {O}nce established, cultures proliferated for 12 months and genomic {DNA} was sampled at 4 months intervals for the estimation of global {DNA} methylation rates through high performance liquid chromatography ({HPLC}) quantitation of deoxynucleosides. {O}ur results show that in vitro proliferation induces {DNA} hypermethylation in a time-dependent fashion. {M}oreover, this trend is statistically significant in several clonal lines and shared between subclonal lines originating from the same genotype. {I}nterestingly, the only clonal line undergoing loss of genomic methylation in the course of proliferation has been found unable to generate somatic embryos. {W}e discuss the possible implications of genome-wide {DNA} methylation changes in proliferating cells with a view to the maintenance of genomic and epigenomic stability.},
  keywords = {{A}recaceae ; {DNA} methylation ; {E}laeis guineensis ; {E}pigenetic stability ; {S}omaclonal variation ; {S}omatic embryogenesis},
  booktitle = {},
  journal = {{P}lant {C}ell {R}eports},
  volume = {32},
  numero = {3},
  pages = {359--368},
  ISSN = {0721-7714},
  year = {2013},
  DOI = {10.1007/s00299-012-1369-y},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058994},
}