@article{fdi:010058946,
  title = {{F}ield evaluation of rapid diagnostic tests for malaria in {Y}aounde, {C}ameroon},
  author = {{T}ahar, {R}achida and {S}ayang, {C}. and {F}oumane, {V}. {N}. and {S}oula, {G}. and {M}oyou-{S}omo, {R}. and {D}elmont, {J}. and {B}asco, {L}eonardo},
  editor = {},
  language = {{ENG}},
  abstract = {{R}apid diagnostic tests ({RDT}s) are affordable, alternative diagnostic tools. {T}he present study aimed to evaluate {RDT}s available in {C}ameroon and compare their characteristics to follow the parasitological response of patients for 28 days. {M}alaria diagnosis was assessed in 179 febrile patients using conventional microscopy as the reference method. {P}arascreen detects both {P}lasmodium falciparum-specific histidine-rich protein 2 ({P}f {HRP}-2) and {P}an-specific plasmodial lactate dehydrogenase (p{LDH}) in all four human {P}lasmodium spp. {D}iaspot is based on the detection of {P}f {HRP}-2. {O}pti{MAL}-{IT} (p{LDH} specific for {P}. falciparum and p{LDH} for all four human {P}lasmodium spp.) was assessed for comparison. {T}he reliability of {RDT}s was evaluated by calculating the sensitivity, specificity, positive predictive value, negative predictive value, false-positive rate, false-negative rate, and likelihood ratio. {T}he clinical outcome of 18 children treated with atovaquone-proguanil and followed for 28 days was evaluated using microscopy and {RDT}s. {O}f 179 samples, 133 (74.3%) were pure {P}. falciparum-positive smears, 4 (2.2%) pure {P}. malariae-positive smears, and 42 (23.5%) negative smears. {P}arascreen and {D}iaspot had high sensitivity (>92%) and positive predictive values (>94%). {T}he specificities for {P}arascreen and {D}iaspot were 81.0% and 90.5%, respectively. {T}he false-positive rates and the false-negative rates were 19.0% and 4.5% for {P}arascreen and 9.5% and 8.3% for {D}iaspot, respectively. {M}ost false-negatives occurred in samples with low parasitaemia (<500 asexual parasites/mu {L}). {T}he performance of {RDT}s was better at higher parasitaemia (>500 asexual parasites/mu {L}.). {F}our pure {P}. malariae were only detected by the pan-{P}lasmodium bands of {P}arascreen and {O}pti{MAL}-{IT}. {I}n blood samples from patients treated and followed-up for 28 days, {HRP}2-based {RDT}s remained positive in most samples until {D}ay 28. {D}espite negative smears, {O}pti{MAL}-{IT} remained positive in several patients until {D}ay 7 but was negative in all patients from {D}ay 14 onwards. {RDT}s can improve the management of febrile patients. {T}he validity, ease of use, and cost of {HRP}2-based tests were comparable. {H}owever, one of the current weaknesses of the {RDT}-based strategy using the tests available in {C}ameroon is inadequate sensitivity for low parasitaemia. {I}n some cases, {ROT} results may require correct interpretation based on clinical history, clinical examination, and microscopic diagnosis.},
  keywords = {{M}alaria ; {P}lasmodium falciparum ; {P}lasmodium malariae ; {D}rug resistance ; {D}iagnosis ; {A}tovaquone ; {CAMEROUN}},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {125},
  numero = {2},
  pages = {214--219},
  ISSN = {0001-706{X}},
  year = {2013},
  DOI = {10.1016/j.actatropica.2012.10.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058946},
}