@article{fdi:010058846,
  title = {{M}icrobial community responses to bioremediation treatments for the mitigation of low-dose anthracene in marine coastal sediments of {B}izerte lagoon ({T}unisia)},
  author = {{L}ouati, {H}. and {B}en {S}aid, {O}. and {G}ot, {P}. and {S}oltani, {A}. and {M}ahmoudi, {E}. and {C}ravo-{L}aureau, {C}. and {D}uran, {R}. and {A}issa, {P}. and {P}ringault, {O}livier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he main goals of this study were to investigate (1) the behavior of microbial communities in response to low-dose bioavailable anthracene addition in lightly contaminated sediment from {B}izerte {L}agoon and (2) the effects of bioremediation treatments on microbial biomass, activity, and community structure. {S}ediment microcosms amended with 1 ppm anthracene were incubated in triplicate during 30 days. {B}iostimulation (addition of nitrogen and phosphorus fertilizer) and bioaugmentation (inoculation of a hydrocarbonoclastic bacterium) were used as bioremediation treatments. {B}acterial biomass was estimated using flow cytometry. {S}ediment oxygen consumption was measured with oxygen microelectrodes. {B}acterial community structure was assessed by molecular fingerprints (terminal restriction fragment length polymorphism; {T}-{RFLP}) analysis. {A}nthracene contamination resulted in a significant reduction of bacterial abundance with an impact on cell integrity. {C}oncomitantly, sediment oxygen consumption was strongly inhibited. {C}orrespondence analysis on {T}-{RFLP} data indicated that bacterial community structures from anthracene-contaminated microcosms were different from that of the control. {I}nterestingly, the changes observed in microbial biomass, structure, and activities as a result of anthracene contamination were not alleviated even with the use of biostimulation and combination of biostimulation and bioaugmentation strategy for anthracene bioremediation. {N}evertheless, both treatment methods resulted in different community structures relative to the contaminated and control microcosms with the appearance of distinct populations. {A}nthracene spiking severely affected microbial communities, suggesting dominance of nontolerant populations in this lightly-contaminated sediment. {A}lthough biostimulation and/or bioaugmentation treatments did not alleviate the anthracene toxic effects, the changes observed in microbial population and structure suggest that the proposed treatments might be promising to promote bacterial growth. {F}urther works are still required to propose a more efficient strategy to stimulate biodegradation that takes into account the complex interactions between species for resource access.},
  keywords = {{T}-{RFLP} analysis ; {O}xygen microprofiles ; {A}nthracene ; {M}icrocosms ; {B}izerte ; lagoon ; {M}icrobial communities ; {TUNISIE}},
  booktitle = {},
  journal = {{E}nvironmental {S}cience and {P}ollution {R}esearch},
  volume = {20},
  numero = {1},
  pages = {300--310},
  ISSN = {0944-1344},
  year = {2013},
  DOI = {10.1007/s11356-012-0860-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058846},
}