@article{fdi:010057297,
  title = {{M}ass spectrometry detection of {G}3m and {IGHG}3 alleles and follow-up of differential mother and neonate {I}g{G}3},
  author = {{D}echavanne, {C}{\'e}lia and {G}uillonneau, {F}. and {C}hiappetta, {G}. and {S}ago, {L}. and {L}evy, {P}. and {S}alnot, {V}. and {G}uitard, {E}. and {E}hrenmann, {F}. and {B}roussard, {C}. and {C}hafey, {P}. and {L}e {P}ort, {A}. and {V}inh, {J}. and {M}ayeux, {P}. and {D}ugoujon, {J}. {M}. and {L}efranc, {M}. {P}. and {M}igot {N}abias, {F}lorence},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ass spectrometry ({MS}) analysis for detection of immunoglobulins ({IG}) of the human {I}g{G}3 subclass is described that relies on polymorphic amino acids of the heavy gamma3 chains. {I}g{G}3 is the most polymorphic human {I}g{G} subclass with thirteen {G}3m allotypes located on the constant {CH}2 and {CH}3 domains of the gamma3 chain, the combination of which leads to six major {G}3m alleles. {A}mino acid changes resulting of extensive sequencing previously led to the definition of 19 {IGHG}3 alleles that have been correlated to the {G}3m alleles. {A}s a proof of concept, {MS} proteotypic peptides were defined which encompass discriminatory amino acids for the identification of the {G}3m and {IGHG}3 alleles. {P}lasma samples originating from ten individuals either homozygous or heterozygous for different {G}3m alleles, and including one mother and her baby (drawn sequentially from birth to 9 months of age), were analyzed. {T}otal {I}g{G}3 were purified using affinity chromatography and then digested by a combination of {A}sp{N} and trypsin proteases, and peptides of interest were detected by mass spectrometry. {T}he sensitivity of the method was assessed by mixing variable amounts of two plasma samples bearing distinct {G}3m allotypes. {A} label-free approach using the high-performance liquid chromatography ({HPLC}) retention time of peptides and their {MS} mass analyzer peak intensity gave semi-quantitative information. {Q}uantification was realized by selected reaction monitoring ({SRM}) using synthetic peptides as internal standards. {T}he possibility offered by this new methodology to detect and quantify neo-synthesized {I}g{G} in newborns will improve knowledge on the first acquisition of antibodies in infants and constitutes a promising diagnostic tool for vertically-transmitted diseases.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {7},
  numero = {9},
  pages = {e46097 [15 ]},
  ISSN = {1932-6203},
  year = {2012},
  DOI = {10.1371/journal.pone.0046097},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057297},
}