@article{fdi:010057048,
  title = {{M}olecular detection of eukaryotes in a single human stool sample from {S}enegal},
  author = {{H}amad, {I}. and {S}okhna, {C}heikh and {R}aoult, {D}idier and {B}ittar, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}icrobial eukaryotes represent an important component of the human gut microbiome, with different beneficial or harmful roles; some species are commensal or mutualistic, whereas others are opportunistic or parasitic. {T}he diversity of eukaryotes inhabiting humans remains relatively unexplored because of either the low abundance of these organisms in human gut or because they have received limited attention from a whole-community perspective. {M}ethodology/{P}rincipal {F}inding: {I}n this study, a single fecal sample from a healthy {A}frican male was studied using both culture-dependent methods and extended molecular methods targeting the 18{S} r{RNA} and {ITS} sequences. {O}ur results revealed that very few fungi, including {C}andida spp., {G}alactomyces spp., and {T}richosporon asahii, could be isolated using culture-based methods. {I}n contrast, a relatively a high number of eukaryotic species could be identified in this fecal sample when culture-independent methods based on various primer sets were used. {A} total of 27 species from one sample were found among the 977 analyzed clones. {T}he clone libraries were dominated by fungi (716 clones/977, 73.3%), corresponding to 16 different species. {I}n addition, 187 sequences out of 977 (19.2%) corresponded to 9 different species of plants; 59 sequences (6%) belonged to other micro-eukaryotes in the gut, including {E}ntamoeba hartmanni and {B}lastocystis sp; and only 15 clones/977 (1.5%) were related to human 18{S} r{RNA} sequences. {C}onclusion: {O}ur results revealed a complex eukaryotic community in the volunteer's gut, with fungi being the most abundant species in the stool sample. {L}arger investigations are needed to assess the generality of these results and to understand their roles in human health and disease.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {7},
  numero = {7},
  pages = {e40888},
  ISSN = {1932-6203},
  year = {2012},
  DOI = {10.1371/journal.pone.0040888},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057048},
}