@article{fdi:010057037,
  title = {{S}ensitivity of {T}richodesmium erythraeum and {C}rocosphaera watsonii abundance and {N}-2 fixation rates to varying {NO}3- and {PO}43- concentrations in batch cultures},
  author = {{K}napp, {A}. {N}. and {D}ekaezemacker, {J}. and {B}onnet, {S}ophie and {S}ohm, {J}. {A}. and {C}apone, {D}. {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}atch cultures of {T}richodesmium erythraeum, strain {IMS}101, and {C}rocosphaera watsonii, strain {WH}8501, were grown under metal- and vitamin-replete conditions to evaluate differences in diazotroph abundance and {N}-2 fixation rates as well as biomass {C}:{N}:{P} ratios resulting from changes in the concentrations of nitrate ({NO}3-) and phosphate ({PO}43-) in culture media. {H}olding light levels and temperature constant, variations in culture {NO}3- and {PO}43- concentrations included ({N}:{P} ratios in mu {M}) 0:0.5, 5:1, 8:0.5, and 16:1. {T}he abundance of both diazotrophs was greatest in the 16:1 and 5:1 {N}:{P} ratio treatments (i.e. those grown with 1 mu {M} {PO}43-) while the highest {N}-2 fixation rates for both diazotrophs were observed in the 0:0.5 treatment (i.e. those grown in {NO}3--free media). {M}easurable but reduced (similar to 25 to 50% of the rates in cultures grown with no {NO}3-) {N}-2 fixation rates were evident in both {T}. erythraeum and {C}. watsonii cultures grown with up to 16 mu {M} {NO}3-. {T}hese results indicate that while diazotrophs grown in the presence of {NO}3- have significantly lower {N}-2 fixation rates than those not chronically exposed to {NO}3-, these lower per cell {N}-2 fixation rates are compensated for by a greater abundance of diazotrophs in treatments with 1 mu {M} {PO}43- and result in comparable volume-integrated rates of {N}-2 fixation. {A}dditionally, {N}-2 fixation rates for {T}. erythraeum and {C}. watsonii were comparable when normalized to carbon (biomass). {F}inally, the exponential-phase {C}:{N}:{P} biomass ratios of both diazotrophs were similar to each other as well as to previous studies and varied little among the treatments but increased, often significantly, between exponential and stationary growth phases.},
  keywords = {{MICROORGANISME} ; {CULTURE} {IN} {VITRO} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {ABONDANCE} ; {BIOMASSE} ; {INHIBITION} ; {FACTEUR} {LIMITANT} ; {NITRATE} ; {PHOSPHATE} ; {CYANOBACTERIE} ; {RATIO} {CNP} ; {N}-2 fixation ; {T}richodesmium ; {C}rocosphaera ; {I}nhibition ; {C}:{N}:{P} ratio},
  booktitle = {},
  journal = {{A}quatic {M}icrobial {E}cology},
  volume = {66},
  numero = {3},
  pages = {223--236},
  ISSN = {0948-3055},
  year = {2012},
  DOI = {10.3354/ame01577},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057037},
}