@article{fdi:010057026,
  title = {{D}evelopment of {E}xon-{P}rimed {I}ntron-{C}rossing ({EPIC}) {PCR} primers for the malaria vector {A}nopheles pseudopunctipennis ({D}iptera : {C}ulicidae)},
  author = {{L}ardeux, {F}r{\'e}d{\'e}ric and {A}liaga, {C}. and {T}ejerina, {R}. and {U}rsic-{B}edoya, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{U}sing the {A}nopheles gambiae {G}iles genome as a template, we designed, screened and identified 14 novel {E}xon-{P}rimed {I}ntron-{C}rossing ({EPIC}) {PCR} primer pairs for {A}nopheles pseudopunctipennis {T}heobald 1901, a major vector of human {P}lasmodium sp. in {S}outh {A}merica. {T}hese primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of 17 loci of which nine were polymorphic. {P}olymorphisms at these loci ranged from two to four alleles. {I}ntron length polymorphism analysis is a useful tool, which will allow the study of the population structure of this mosquito species, which remains poorly understood.},
  keywords = {{EPIC}-{PCR} ; {A}nopheles pseudopunctipennis ; {M}alaria ; {P}rimer ; {I}ntron},
  booktitle = {},
  journal = {{C}omptes {R}endus {B}iologies},
  volume = {335},
  numero = {6},
  pages = {398--405},
  ISSN = {1631-0691},
  year = {2012},
  DOI = {10.1016/j.crvi.2012.05.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057026},
}